Comparing the efficiency of agar-plate culture in the fieldwork and laboratory to diagnose Strongyloides stercoralis infection
Keywords:
Strongyloides stercoralis, Agar plate culture, Formalin-ethyl acetate concentration, Enzyme-linked immunosorbent assaysAbstract
Strongyloides stercoralis is an intestinal nematode that causes strongyloidiasis, a parasitic infection that is distributed in tropical and temperate climates, including the northeast region of Thailand. The infection is acquired by skin penetration of infective filariform larvae while walking barefoot over contaminated soil. Strongyloides stercoralis is able to maintain itself for decades within its host and may cause lethal hyperinfection syndrome among immune-compromized patients. In the present study, we compared the efficacy of agar plate culture technique (APCT) between in the fieldwork and laboratory, as well as compared to other parasitological methods include urine enzyme-linked immunosorbent assays (urine-ELISA) and formalin-ethyl acetate concentration (FECT). A total of 103 stool samples from Nong Kung Sri Districts in Kalasin Province were examined by APCT in the fieldwork and laboratory. The prevalence of S. stercoralis from APCT in the fieldwork was 29.1% and 17.5% in the laboratory. The APCT in the fieldwork for the detection of S. stercoralis had high sensitivity (72.2%) than APCT in the laboratory and revealed statistically significant difference (P<0.001). The diagnosis of S. stercoralis using APCT was considered the combination of results from 2 different parasitological methods. The urine-ELISA was the most sensitivity for the detection of S. stercoralis (97.1%). The APCT and FECT had sensitivity for the recovery of S. stercoralis as 72.2% and 22.9%, respectively.
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