ผลของสารสกัดจากผลฟักข้าวต่อการเจริญของเชื้อแบคทีเรียประจำถิ่นบนผิวหนัง Staphylococcus epidermidis และยับยั้งการเจริญของเชื้อก่อโรค Staphylococcus aureus

Abstract

Gac fruit (Momordica cochinchinensis (Lour.) Spreng.) is a plant that contained with various kind of importance substances, especially, phenolic and flavonoid compounds, which has antioxidant activity and antimicrobial activity, against pathogenic bacteria. Concurrently, it is beneficial for the growth of probiotics such as Staphylococcus epidermidis, which prevents pathogenic microorganisms on the skin. This study is aimed to determine the total phenolic and total flavonoids compounds of gac fruit extract, including the growth-promoting effect of S. epidermidis, which affects to inhibit of the growth of Staphylococcus aureus. The results revealed that the highest total phenolic content found in the peel extract (29.17 ± 0.28 mg GAE/g DW) and highest flavonoid content found in the aril extract (18.28 ± 0.89 mg QE/g DW). It was found that the extracts of the peel had the highest antibacterial activity, with the minimum inhibitory concentration at 50.00 mg/ml and the minimum bactericidal concentration at 100.00 mg/ml. The growth-promoting effect of S. epidermidis, the peel extracts had been used. At the concentration as 12.50 mg/ml can promote the growth of S. epidermidis similar to the control group. In addition, the result indicated that the highest antibacterial control was observed when used cell free supernatant from this condition to co-culture with S. aureus (10.37 ± 0.31 log colonies/ml). This discovery indicates the benefits of gac fruit in terms of controlling skin pathogens and might be further developed for the development of healthy skin care products in the future.