Comparing the Efficiency of Agar Plate Culture for Diagnosing Strongyloides Stercoralis Infection in Field and Laboratory Settings


  • Wansiri Wiraphongthongchai Master of Science Program in Parasitology, Department of Parasitology, Faculty of Medicine, Khon Kaen University and Cholangiocarcinoma Research Institute (CARI), Khon Kaen University Email:
  • Paiboon Sithithaworn
  • Apiporn Suwannatrai Department of Parasitology, Faculty of Medicine, Khon Kaen University


Strongyloides stercoralis, Agar plate culture, Formalin-ethyl acetate concentration, Enzyme-linked immunosorbent assays


Strongyloidiasis, caused by the intestinal nematode Strongyloides stercoralis, is a parasitic infection that is endemic in tropical and temperate regions, including the northeast of Thailand. In this study, we aimed to compare the efficacy of agar plate culture technique (APCT) for detecting S. stercoralis between field and laboratory settings, as well as compared to other parasitological methods including urine enzyme-linked immunosorbent assays (urine-ELISA) and formalin-ethyl acetate concentration (FECT). A total of 103 stool samples were collected from Nong Kung Sri Districts in Kalasin Province and examined by APCT in both field and laboratory settings. The prevalence of S. stercoralis was higher in the field (29.13%) than in the laboratory (17.43%) (P≤0.001). The diagnosis of S. stercoralis using APCT was considered the combination of results from two different parasitological methods. The urine-ELISA was the most sensitive method for detecting S. stercoralis (100%, 95%CI: 100), while FECT had low sensitivity for the recovery of S. stercoralis (26.67%, 95%CI: 18.13-35.21) compared to APCT (43.33%, 95%CI: 33.76-52.90). These findings suggest that APCT is a useful method for diagnosing S. stercoralis infection, especially in field settings, while urine-ELISA may be a promising alternative method for detecting the infection.


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