In Vitro Point-of-Care Diagnostic Assays for SARS-CoV-2 (COVID-19)
In Vitro Point-of-Care Diagnostic Assays for COVID-19
Keywords:
Coronavirus disease 2019 (COVID-19), point-of-care detection kit, in vitro diagnosisAbstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes the currently pandemic contagious coronavirus disease 2019 known as COVID-19 disease in Thailand and worldwide. Development of diagnostic methods to reach people at the regional and local settings quickly and inexpensively are thereby necessary to help stop the spread of the COVID-19 infected people. The standard conventional diagnosis of COVID-19 in laboratory is using reverse transcription followed quantitative polymerase chain reaction (RT-qPCR) techniques, which is specific and accurate but requires a high-priced analytical equipment and long-time analysis. Therefore, the invention and development of the SARS-CoV-2 (COVID-19) test kit for the local and point-of-care detection will help resolve these problems. Now, the COVID-19 test kits are classified by the detection of viral genetic material in respiratory secretions, and the detection of human blood immunity (immunoglobulin: Ig). This article presents information and details about these COVID-19 test kits, including limitations and ongoing researches for the more effective COVID-19 test ki
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17. Heid CA, Stevens J, Livak KJ, Williams PM. Real time quantitative PCR. Genome Res 1996;6(10):986-94.
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26. Zhang F, Abudayyeh OO, Gootenberg JS. A protocol for detection of COVID-19 using CRISPR diagnostics [online]. 2020 [cited 2020 May 1]. Available from: https://www.broadinstitute.org/files/publications/special/COVID-19%20detection%20
(updated).pdf
27. Broughton JP, Deng X, Yu G, Fasching CL, Singh J, Streithorst J, et al. Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR-based DETECTR lateral flow assay. medRxiv 2020. doi:10.1101/2020.03.06.20032334
28. Adams ER, Anand R, Andersson MI, Auckland K, Baillie JK, Barnes E, et al. Evaluation of antibody testing for SARS-Cov-2 using ELISA and lateral flow immunoassays.medRxiv 2020. doi:10.1101/2020.04.15.20066407
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31. Li Z, Yi Y, Luo X, Xiong N, Liu Y, Li S, et al. Development and clinical application of a rapid IgM-IgG combined antibody test for SARS-CoV-2 infection diagnosis. J Med Virol 2020. doi:10.1002/jmv.25727
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34. Lv H, Wu NC, Tsang OTY, Yuan M, Perera RA, Leung WS, et al. Cross-reactive antibody response between SARS-CoV-2and SARS-CoVinfections. bioRxiv 2020.doi:10.1101/2020.03.15.993097
35. To KKW, Tsang OTY, Leung WS, Tam AR, Wu TC, Lung DC, et al. Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: An observational cohort study. Lancet Infect Dis2020. doi:10.1016/S1473-3099(20)30196-1
36. Yan C, Cui J, Huang L, Du B, Chen L, Xue G, et al. Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated is othermal amplification assay. Clin Microbiol Infect 2020. doi:10.1016/j.cmi.2020.04.001
37. Zhao J, Yuan Q, Wang H, Liu W, Liao X, Su Y, et al. Antibody responses to SARSCoV-2 in patients of novel coronavirus disease 2019. Clin Infect Dis 2020. doi:10.1093/cid/ciaa344
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2020-09-24
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