Acetylcholinesterase Inhibitory Activities of Berries Cultivars in Thailand
Keywords:
Alzheimer’ disease, Acetylcholinesterase inhibition, Berries cultivars in ThailandAbstract
Alzheimer's disease is a brain disorder affecting memory, thinking skills, behaviors and emotions, the most prevalent form of dementia with an increasing tendency among elderly people. Globally, over 50 million people present the disease. Consuming foods rich in vitamins, minerals and phytochemicals in plants can reduce acetylcholinesterase, which is a major cause of Alzheimer's disease. Berries constitute fruits that can be found locally in all parts of Thailand comprising a rich source of various phytochemicals including flavonoids, carotenoid phenolics and alkaloids. The purpose of this study was to determine the acetylcholinesterase inhibitory activities of ten Thai berries. The results showed that ma-kham pom, ma-mao, ma-kiang, and ma-yom with 3 mg/ml extract exhibited the highest anti-acetylcholinesterase activities ranging from 89 to 91% inhibition. In addition, Ma-kham-pom exhibited the highest quantity of Total phenolic content (TPC) and antioxidant activities as being detected by Ferric reducing antioxidant power (FRAP) and Oxygen radical absorbance capacity (ORAC) assay. The results of this study revealed that berries cultivars in Thailand provided efficacy in antioxidant activities, inhibiting acetylcholinesterase leading to the development of dietary supplements to prevent and treat Alzheimer’s disease.
References
ก้องเกียรติ กูณฑ์กันทรากร. (2561). ภาวะสมองเสื่อมและโรคอัลไซเมอร์. ธรรมศาสตร์เวชสาร, 18(3),442-468.
สุพัฒน์ หลังยาหน่าย, ประภาพร จันทร์เอียด, & จินดาพร ภูริพัฒนาวงษ์. (2560). ฤทธิ์ต้านเอนไซม์อะซิทิลโคลีนเอสเทอเรสและต้าน
อนุมูลอิสระของสมุนไพรไทยบางชนิดในวงศ์ Asteraceae และ Cucurbitaceae. วารสารวิทยาศาสตร์ มศว., 33(1), 35-45.
Ainsworth, E. A, & Gillespie, K. M. (2007) Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. Nature Protocols, 2, 875-877.
Almeida, M. M. B., De Sousa, P. H. M., Arriaga, Â. M. C., Do Prado, G. M., de Carvalho Magalhães, C. E., Maia, G.A., et al. (2011). Bioactive compounds and antioxidant activity of fresh exotic fruits from northeastern Brazil. Canadian Institute of Food Science and Technology, 44, 2155-2159.
Alzheimer’s Disease International. (2015). World alzheimer report 2015. London: Alzheimer’s Disease International.
Barbosa-Filho, J. M., Medeiros, K. C. P., Diniz, M. F., Batista, L. M., Athayde-Filho, P. F., Silva, M. S., et al. (2006). Natural products inhibitors of the enzyme acetylcholinesterase. Revista brasileira de farmacognosia, 16, 258-285.
Benzie, I. F., & Strain, J. J. (1996) The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Journal of Biochemistry and Analytical Studies, 239, 70-76.
Dhale, D. A., & Mogle, U. P. (2011). Phytochemical screening and antibacterial activity of Phyllanthus emblica (L.). Journal of Scientific Research and Reports, 1, 138-142.
Duangporn, P., & Siripong, P. (2009). Effect of auxin and cytokinin on phyllanthusol A production by callus cultures of Phyllanthus acidus skeels. American-Eurasian Journal of Agricultural & Environmental Sciences, 5, 258-263.
Hyesung, H., Young, J. K., & Youngjae, S. (2020). Assessment of physicochemical quality, antioxidant content and activity, and inhibition of cholinesterase between unripe and ripe blueberry fruit. Foods, 9, 690.
Jung, H. A., Min, B. S., Yokozawa, T., Lee, J. H., Kim, Y. S., & Choi, J. S. (2009). Anti-alzheimer and antioxidant activities of Coptidis Rhizoma alkaloids. Biological & Pharmaceutical Bulletin, 32, 1433-1438.
Kaewkaen, P., Tong-un, T., Wattanathorn, J., Muchimapura, S., Kaewrueng, W., & Wongcharoenwanakit, S. (2012). Mulberry fruit extract protects against memory impairment and hippocampal damage in animal model of vascular dementia. Evidence-Based Complementary and Alternative Medicine, 1-9.
Kregel, K. C., & Zhang, H. J. (2007). An integrated view of oxidative stress in aging: basic mechanisms, functional effects, and pathological considerations. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 292, 18-36.
Letenneur, L., Proust-Lima, C., Le Gouge, A., Dartigues, J. G., & Barberger-Gateau, P. (2007). Flavonoid intake and cognitive decline over a 10-year period. American Journal of Epidemiology, 65, 1364-1371.
Li, W., Hydamaka A. W., Lowry, L., & Beta, T. (2009). Comparison of antioxidant capacity and phenolic compounds of berries, chokecherry and seabuckthorn. Central European Journal of Biology, 4, 499-506.
Liu, X., Zhao, M., Wang, J., Yang, B., & Jiang, Y. (2008). Antioxidant activity of methanolic extract of emblica fruit (Phyllanthus emblica L.) from six regions in China. Journal of Food Composition and Analysis, 21, 219-228.
Moniruzzaman, M., Asaduzzaman, M., Hossain, M. S., Sarker, J., Rahman, S. A., Rashid, M., et al. (2015). In vitro antioxidant and cholinesterase inhibitory activities of methanolic fruit extract of Phyllanthus acidus. BMC Complementary and Alternative Medicine, 15, 403.
Ou, B., Hampsch-Woodill, M., & Prior, R. L. (2001). Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. Journal of Agricultural and Food Chemistry, 49, 4619-4626.
Radojkovi, M. M., Zekovi, Z. P., Vidovi, S. S., Kočar, D. D., & Maškovi, P. Z. (2012). Free radical scavenging activity and total phenolic and flavonoid contents of mulberry (Morus spp. L., Moraceae) extracts. Hemijska Industrija, 66, 547–552.
Robert, K., Marcelle, D. S., Tiffany, A. N., Wilhelmina, K., Melinda, R. V. T., Barbara, S. H., et al. (2010). Blueberry Supplementation Improves Memory in Older Adults. Journal of Agricultural and Food Chemistry, 58(7), 3996-4000.
Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144-158.
Wang, S. Y., & Lin, H. S. (2000). Antioxidant activity in fruits and leaves of blackberry, raspberry, and strawberry varies with cultivar and developmental stage. American Chemical Society, 48, 140-146.
Zheng, W., & Wang, Y. S. (2001). Antioxidant Activity and Phenolic Compounds in Selected Herbs. Journal of Agricultural and Food Chemistry, 49, 5165-5170.
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