Establishment of Methyl Anthranilate Quantification by UV-Spectroscopy and Identification Using FTIR

Abstract

The objective of this research was to develop the extraction method of methyl anthranilate from herbal (medicinal) plants ginger, galangal, and lemongrass, with 2 types of solvents, 1% (v/v) sulfuric acid in methanol and 1 M sulfuric acid in water. The absorbtion  of the extracts were measured and compared to the standard curve of methyl anthranilate with a wavelength of 273 nanometers. The study found the extractions with the two methods yielded parallel results. And the highest amount of methyl anthranilate of galangal, lemon grass and ginger with 1%(v/v) sulfuric acid in methanol were 6.813, 1.349 and 0.606 %w/w fresh plant, respectively. The accuracy resulted in the average percentage of recovery from between 97.2-104.3% and the precision result in the percentage of the relative standard deviation (%RSD) with the concentration 30 mgL^-1 were 3.6, 3.3 and 0.8% of ginger, galangal and lemongrass, respectively. The results of the percentage yield analysis of the methyl anthranilate with the diazotization reaction showed that the standard methyl anthranilate substance was 58.97% and was sufficient to prove the identity of the diazotization reaction with the FTIR technique