Establishment of Methyl Anthranilate Quantification by UV-Spectroscopy and Identification Using FTIR

Main Article Content

Ruchira Khoomsab
Nantharak Rodkate

Abstract

The objective of this research was to develop the extraction method of methyl anthranilate from herbal (medicinal) plants ginger, galangal, and lemongrass, with 2 types of solvents, 1% (v/v) sulfuric acid in methanol and 1 M sulfuric acid in water. The absorbtion  of the extracts were measured and compared to the standard curve of methyl anthranilate with a wavelength of 273 nanometers. The study found the extractions with the two methods yielded parallel results. And the highest amount of methyl anthranilate of galangal, lemon grass and ginger with 1%(v/v) sulfuric acid in methanol were 6.813, 1.349 and 0.606 %w/w fresh plant, respectively. The accuracy resulted in the average percentage of recovery from between 97.2-104.3% and the precision result in the percentage of the relative standard deviation (%RSD) with the concentration 30 mgL-1 were 3.6, 3.3 and 0.8% of ginger, galangal and lemongrass, respectively. The results of the percentage yield analysis of the methyl anthranilate with the diazotization reaction showed that the standard methyl anthranilate substance was 58.97% and was sufficient to prove the identity of the diazotization reaction with the FTIR technique

Downloads

Download data is not yet available.

Article Details

Section
บทความวิจัย