Comparison Quality of Wright-Giemsa stain between Modified one step staining method and Commercial stain kit

Abstract

This study aimed to compare the staining quality of a modified one-step staining method with a commercial Wright-Giemsa stain kit. Peripheral blood smears were prepared from 150 individuals at Singburi Hospital, comprising an equal distribution of normal and abnormal cases. Each smear was stained using both methods and subsequently evaluated under a light microscope by two medical technologists. Statistical analysis was performed using a paired sample t-test to compare the efficacy of the two methods.

The results indicated no statistically significant difference in staining quality between the two methods (p > 0.05). The mean scores for the modified one-step method and the commercial kit were 4.92 ±0.28 and 4.93 ± 0.26   respectively. Notably, the modified one-step method required only 7 minutes and a single step to complete, whereas the commercial kit took 8 minutes and involved a three-step process. Consequently, the modified method significantly reduced the laboratory workload. Furthermore, the preparation cost for the modified one-step stain was approximately three times lower than that of the commercial kit.

In conclusion, the modified one-step staining method is a viable alternative for routine hematological work due to its simplified procedure, cost-effectiveness, and high-quality results.