การพัฒนาผลิตภัณฑ์บาล์มที่มีส่วนผสมของสารสกัดพิกัดเบญจเทียน
คำสำคัญ:
บาล์ม, พิกัดเบญจเทียน, ฤทธิ์ทางชีวภาพบทคัดย่อ
การวิจัยครั้งนี้มีวัตถุประสงค์เพื่อพัฒนาผลิตภัณฑ์บาล์มที่มีส่วนผสมของสารสกัดพิกัดเบญจเทียน โดยศึกษาสารพฤกษเคมีเบื้องต้น และศึกษาฤทธิ์ต้านอนุมูลอิสระของสารสกัดพิกัดเบญจเทียน ประกอบด้วย เมล็ดเทียนดำเทียนแดง เทียนขาว เทียนข้าวเปลือก และเทียนตาตั๊กแตน จากการศึกษาสารพฤกษเคมีเบื้องต้นด้วยวิธีการอาศัยปฏิกิริยาการเกิดสีหรือตะกอน พบสารกลุ่มอัลคาลอยด์ คาดิแอคไกลโคไซต์ คูมาริน ฟลาโวนอยด์ สเตอรอยด์ แทนนิน และเทอร์ปินอยด์ ศึกษาฤทธิ์ต้านอนุมูลอิสระด้วยวิธี DPPH radical scavenging และวิธี ABTS radical cation decolorization พบว่า เทียนแดง มีค่า IC50 สูงที่สุด เท่ากับ 30.31 และ 76.90 ไมโครกรัมต่อมิลลิลิตร ตามลำดับ เทียนขาวมีปริมาณฟีนอลิกรวมและฟลาโวนอยด์รวมมากที่สุด เท่ากับ 58.51±1.78 มิลลิกรัมแกลลิกต่อกรัมตัวอย่าง และ 85.71±3.80 มิลลิกรัมเควอซิตินต่อกรัมตัวอย่าง ตามลำดับ จากผลการศึกษาเบื้องต้น จึงนำสารสกัดพิกัดเบญจเทียนมาเป็นส่วนผสมของผลิตภัณฑ์บาล์ม โดยมีสัดส่วนที่ความเข้มข้นร้อยละ 0.5 ทดสอบคุณลักษณะทางกายภาพของบาล์มเป็นไปตามเกณฑ์มาตรฐานสำหรับผลิตภัณฑ์ ดังนั้นบาล์มที่มีน้ำมันหอมระเหยและฤทธิ์ต้านอนุมูลอิสระสามารถพัฒนาเป็นผลิตภัณฑ์ได้หลากหลาย ซึ่งในอนาคตสามารถนำผลิตภัณฑ์บาล์มนี้ไปพัฒนาต่อยอดในระดับอุตสาหกรรมต่อไปได้
เอกสารอ้างอิง
Agatonovic-Kustrin, S., Kustrin, E., Gegechkori, V., & Morton, D. W. (2020). Anxiolytic terpenoids and aromatherapy for anxiety and depression. Advances in experimental medicine and biology, 1260, 283–296. https://doi.org/10.1007/978-3-030-42667-5_11
Arnao, M. B., Cano, A., & Acosta, M., (2001). The hydrophilic and lipophilic contribution to total antioxidant activity. Journal of Agricultural and Food Chemistry, 73, 239-244. https://doi.org/10.1016/S0308-8146(00)00324-1
Ashraf, M., Saeed, H., Saleem, Z., Rathore, H. A., Rasool, F., Tahir, E., Bhatti, T., Khalid, J., Bhatti, I., & Tariq, A. (2019). A cross-sectional assessment of knowledge, attitudes and self-perceived effectiveness of complementary and alternative medicine among pharmacy and non-pharmacy university students. BMC Complementary and Alternative Medicine, 19(1), 95. https://doi.org/10.1186/s12906-019-2503-y
Avidan, A. Y., & Neubauer, D. N. (2017). Chronic insomnia disorder. Continuum (Minneapolis, Minn.), 23(4), 1064–1092. https://doi.org/10.1212/01.CON.0000522244.13784.bf
Ayoola, G. A., Coker, H. A. B., Adesegun, S. A., Adepoju-Bello, A. A., Obaweya, K., Ezennia, E. C., & Atangbayila, T. O. (2008) Phytochemical screening and antioxidant activities of some selected medicinal plants used for Malaria Therapy in Southwestern Nigeria. Tropical Journal of Pharmaceutical Research, 7, 1019-1024. https://doi.org/10.4314/tjpr.v7i3.14686
Bahramikia, S., & Yazdanparast, R. (2008). Antioxidant and Free Radical Scavenging Activities of Different Fractions of Anethum Graveolens Leaves Using in Vitro Models. Pharmacologyonline, 2, 219-233. https://pharmacologyonline.silae.it/files/archives/2008/vol2/21_Yazdanparast.pdf
Basavegowda, J., Raveesha, R. A. K., & Amruthesh, K.N. (2022). Bioactivity and Phytochemical Studies of Seed Extracts of Anethum graveolens Linn. Letters in Applied NanoBioScience, 11(2), 3560-3572. https://doi.org/10.33263/LIANBS112.35603572
Beyazen, A., Dessalegn, E., & Mamo, W. (2017). Phytochemical screening, antioxidant and antimicrobial activities of seeds of Foeniculum vulgare (ensilal). World Journal of Pharmaceutical Sciences, 5(3), 198-208. https://www.wjpsonline.com/index.php/wjps/article/view/phytochemical-screening-antioxidant-foeniculum-vulgare
Braca, A., Sortino, C., Politi, M., Morelli, I., & Mendez, J. (2002). Antioxidant activity of flavonoids from Licania licaniaeflora. Journal of Ethnopharmacology, 79(3), 379–381. https://doi.org/10.1016/s0378-8741(01)00413-5
Brown, R. E., Basheer, R., McKenna, J. T., Strecker, R. E., & McCarley, R. W. (2012). Control of sleep and wakefulness. Physiological Reviews, 92(3), 1087–1187. https://doi.org/10.1152/physrev.00032.2011
Bueraheng, S., & Petchlert, C. (2015). Antioxidant activity of ethanol extracts from Nigella sativa and Cuminum cyminum L. In Proceedings The 7th National Science Research Conference (pp.1-6). Phitsanulok: Naresuan University. (in Thai)
Cox-Georgian, D., Ramadoss, N., Dona, C., & Basu, C. (2019). Therapeutic and medicinal uses of terpenes. Medicinal Plants: From Farm to Pharmacy, 12, 333–359. https://doi.org/10.1007/978-3-030-31269-5_15
Department of Mental Health, Ministry of Public Health. (2023). Insomnia is related to the body clock and can be resolved without medication. Retrieved from https://dmh.go.th. (in Thai)
Department of Medical Sciences, Ministry of Public Health. (1998). General Traditional Thai Medicine Textbook. Nonthaburi: Department of medical sciences, ministry of public health (in Thai)
Domea, Y., (2018). The development of Wan Roy Pad Balm products in the enterprise development of herbs Klong Luang district, Klong Luang district Pathumthani. VRU Research and Development Journal Science and Technolog, 13(2), 64-73. https://so06.tci-thaijo.org/index.php/vrurdistjournal/article/view/143375/106138 (in Thai)
El-Serehy, H.A., Al-Misned, F.A., Irshad, R., & Ismail, M.M., (2016). ln Vitro Antioxidant and Anti-herpes Activities of Cuminum cyminum Seeds Extract. Biomedical Research, 27(4), 1255-1260. https://www.alliedacademies.org/articles/ln-vitro-antioxidant-and-antiherpes-activities-of-cuminum-cyminum-seeds-extract.pdf
Faculty of Pharmaceutical Sciences, Ubon Ratchathani University. (2022). Herbal medicine database Faculty of Pharmaceutical Sciences Ubon Ratchathani University. Retrieved from https://phar.ubu.ac.th/main/database. (in Thai)
Frass, M., Strassl, R. P., Friehs, H., Mullner, M., Kundi, M., & Kaye, A. D. (2012). Use and Acceptance of Complementary and Alternative Medicine Among the General Population and Medical Personnel. Ochsner Journal, 12(1), 45–56. https://www.ochsnerjournal.org/content/12/1/45
Ghasemzadeh, A., Jaafar, H.Z. & Rahmat, A. (2015). Phytochemical constituents and biological activities of different extracts of Strobilanthes crispus (L.) Bremek leaves grown in different locations of Malaysia. BMC Complementary and Alternative Medicine, 15, 422. https://doi.org/10.1186/s12906-015-0873-3
Gomes de Melo, J., de Sousa Araújo, T. A., Thijan Nobre de Almeida e Castro, V., Lyra de Vasconcelos Cabral, D., do Desterro Rodrigues, M., Carneiro do Nascimento, S., Cavalcanti de Amorim, E. L., & de Albuquerque, U. P. (2010). Antiproliferative activity, Antioxidant Capacity and Tannin Content in Plants of Semi-arid Northeastern Brazil. Molecules (Basel, Switzerland), 15(12), 8534–8542. https://doi.org/10.3390/molecules15128534
Jaengklang, C., Duanyai, S., Somee, W., Thomtakhob, S., & Saengsai, P. (2022). Study on total phenolic content, total flavonoid content and free radical scavenging activity in herbal extracts. Journal of Traditional Thai Medical Research, 8(1), 93-105. https://he02.tci-thaijo.org/index.php/ttm/article/view/255806 (in Thai)
Kazemi, N., Kahrizi, D., Mansouri, M., Karim, H., Vaziri, S., Zargooshi, J., Khanahmadi, M., Shokrinia, M., & Mohammad, N. (2016). Induction of Linalool as a Pharmaceutical and Medicinal Metabolite via Cell Suspension Culture of Cumin (Cuminum cyminum L.). Cellular and Molecular Biology, 62(6), 65-68. https://www.cellmolbiol.org/index.php/CMB/article/view/874/559
Kripke, D. F. (2016). Hypnotic drug risks of mortality, infection, depression, and cancer: But lack of benefit. F1000Research, 5, 918. https://doi.org/10.12688/f1000research.8729.3
Lakshmanan, G., Selvam, R., Altemimi, A. B., Baldelli, A., Bharathi, A. R., Gopalakrishnan, B., Karthik, L., Saravanan, D., Kavitha, M., Mohammed, M. J., Cacciola, F., Jayalakshmi, G., & Pratap-Singh, A. (2023). Phytochemical screening of ethanolic extracts of Cuminum cyminum L. seeds along with the evaluation of antidiabetic properties by molecular docking approach. Natural Product Research, 37(4), 681–686. https://doi.org/10.1080/14786419.2022.2078973
Leproult, R., & Van Cauter, E. (2010). Role of sleep and sleep loss in hormonal release and metabolism. Endocrine Development, 17, 11–21. https://doi.org/10.1159/000262524
Linck, V. M., da Silva, A. L., Figueiró, M., Piato, A. L., Herrmann, A. P., Dupont Birck, F., Caramão, E. B., Nunes, D. S., Moreno, P. R., & Elisabetsky, E. (2009). Inhaled linalool-induced sedation in mice. Phytomedicine: International Journal of Phytotherapy and Phytopharmacology, 16(4), 303–307. https://doi.org/10.1016/j.phymed.2008.08.001
Majhenic, L., Skerget, M., & Knez, Z. (2007). Antioxidant and antimicrobial activity of guarana seed extracts. Food Chemistry, 104(3), 1258-1268. https://doi.org/10.1016/j.foodchem.2007.01.074
Manok, S., & Limcharoen, P. (2015). Investigating antioxidant activity by DPPH, ABTS and FRAP assay and total phenolic Ccompounds of herbal extracts in Ya-Hom Thepphachit. Advanced Science, 15(1), 106-117. https://sci.bsru.ac.th/sciweb/e-magazine/15-1/chapter-10.pdf (in thai)
Mohan, M. E., Thomas, J. V., Mohan, M. C., Das, S. S., Prabhakara, P., & Sasidharan, B.C.P. (2023). A proprietary black cumin oil extract (Nigella sativa) (BlaQmax®) modulates stress-sleep-immunity axis safely: Randomized double-blind placebo-controlled study. Frontiers In Nutrition, 2023, 1-15, https://doi.org/10.3389/fnut.2023.1152680.
Monograph. (2004). Valeriana officinalis. Alternative Medicine Review, 9(4), 438–441. https://altmedrev.com/wp-content/uploads/2019/02/v9-4-438.pdf
Nagoor Meeran, M. F., Javed, H., Taee, H., Al, Azimullah, S., & Ojha, S. K. (2017). Pharmacological properties and molecular mechanisms of thymol: Prospects for its therapeutic potential and pharmaceutical development. Front. Pharmacol, 8, 380. https://doi.org/10.3389/fphar.2017.00380
Niranam. (2010). Saponins, information sources sound towers and information centers science and technology. Retrieved from http://siweb.dss.go.th/repack/fulltext/IR. (in Thai)
Ouariachi, E., El-Lahhit, N., Bouyanzer, A., Hammouti, B., Paolini, J., Majidi, L., Desjobert, J-M., & Costa, J. (2014). Chemical composition and antioxidant activity of essential oils and solvent extracts of Foeniculum Vulgare Mill. from Morocco. Journal of Chemical and Pharmaceutical Research, 6(4), 743-748. https://bit.ly/4l6OxMK
Perry, N., & Perry, E. (2006). Aromatherapy in the management of psychiatric disorders: clinical and neuropharmacological perspectives. CNS Drugs, 20(4), 257–280. https://doi.org/10.2165/00023210-200620040-00001
Philips. (2019). The global pursuit of better sleep health, in global sleep survey, 1-9. Retrieved from https://dmh.go.th. (in Thai)
Sontakke, K. S., Shinde, S. K., More, R. A., Gorathkar, C. K., & Anasane, P.Y. (2019). Preliminary phytochemical analysis, antioxidant and tyrosinase inhibition activities of seeds of Lepidium sativum L. AJANTA, 8, 66-71. https://bit.ly/42ayG7s
Soobrattee, M. A., Neergheen, V. S., Luximon-Ramma, A., Aruoma, O. I., & Bahorun, T. (2005). Phenolics as potential antioxidant therapeutic agents: Mechanism and actions. Mutation Research, 579(1-2), 200–213. https://doi.org/10.1016/j.mrfmmm.2005.03.023
Sultan, N. M, & Katib R. (2021). Evaluation of total phenolic content, total flavonoids content and free radical scavenging activity of Lepidium Sativum L. seeds and leaves planted in Syria. Bulletin of Pharmaceutical Sciences, 44(2), 377-385. https://doi.org/10.21608/BFSA.2021.207156
Tanruean, K., Kaewnarin, K., & Rakariyatham, N. (2014). Antibacterial and antioxidant activities of Anethum graveolens L. dried fruit extracts. Chiang Mai Journal of Science, 41(3), 649-660. https://epg.science.cmu.ac.th/ejournal/journal-detail.php?id=5026 (in Thai)
Thansettakij. (2023). The ‘sleep-seeking market’ is growing rapidly, presenting opportunities for dietary supplements and Thai herbal products. Retrieved from https://www.thansettakij.com/business/economy/575546. (in Thai)
Thongmak, K., Janpat, T., Chandang, R., Marde, W., & Noipha, K. (2021). Preliminary phytochemical study and antioxidant activity of five flowers remedy. Journal of Traditional Thai Medical, 7(2), 61-74. https://he02.tci-thaijo.org/index.php/ttm/article/view/249991/174286 (in Thai)
Vongnoom, C. (2019). Characteristic Identification for Controlling Herbal Materials in Practical Skills in Thai Traditional Pharmacy 1 Subject (Research report). Pathum Thani: Rajamangala University of Technology Thanyaburi. (in Thai)
Wanmanee, N., Siriwattanavorakul, P., Munee, S., Maneechot, A., Marde, W., & Noipha, K. (2021). Phytochemical study, total phenolic, and antioxidant activity of herbal extracts for development of a hand treatment serum. Journal of Traditional Thai Medical Research, 2(7), 31-44. https://he02.tci-thaijo.org/index.php/ttm/article/download/249560/174285/932921 (in Thai)
Welz, A.N., Emberger-Klein, A., & Menrad, K. (2018). Why people use herbal medicine: Insights from a focus-group study in Germany. BMC Complementary Medicine and Therapies, 18, 92. https://doi.org/10.1186/s12906-018-2160-6.
Zhang, H., Liu, P., Wu, X., Zhang, Y., & Cong, D. (2019). Effectiveness of chinese herbal medicine for patients with primary insomnia: A PRISMA-compliant meta-analysis. Medicine (Baltimore), 98(24), e15967. https://doi.org/10.1097/MD.0000000000015967.
