Antioxidant Activity and Tyrosinase Inhibition Activity of Extracts from Elaeocarpus hygrophilus to Cosmetic Formulations for Continue Commercialization
Keywords:
antioxidant activity, tyrosinase inhibition activity, Elaeocarpus hygrophilusAbstract
Elaeocarpus hygrophilus is a common cultivated plant, and its fruit is processed to be fruit preserve. A cosmetic formulation containing E. hygrophilus extract for increasing herbal product value is interesting. This research was conducted to study total phenolic content, total flavonoid content, antioxidant activity, anti-tyrosinase activity, and anti-bacterial activity for all 4 pathogenic bacteria, i.e. S.aureus, E.coli, P.aeruginosa and B.cereus, based on Agar well diffusion method so as to set the formulation of cosmetic products from E. hygrophilus extract. It was found that the total phenolic content of the fruit extract from unripe E. hygrophilus (261.17±0.84 mg GAE/g extract), total flavonoid content (89.19±1.45 mg QE/g extract), antioxidant activity (IC50 equal to 11.29±0.55 µg/ml), and Tyrosinase Inhibition Activity (IC50 equal to 16.10±0.77 µg/ml). Compared to the extract from buds, leaves, and peels, the fruit extract from unripe E. hygrophilus is able to inhibit all 4 pathogenic bacteria, i.e. S.aureus, E.coli, P.aeruginosa and B.cereus, but it can inhibit S.aureus the most with statistical significance at 95% confidence. The fruit extract from unripe E. hygrophilus was used as an ingredient in the formulation of lotion. It was found that the lotion containing the developed fruit extract from unripe E. hygrophilus gave a smooth and fine texture. It has a light yellow, cloudy, opaque, odorless, pH 6.15. The growth of microorganisms and fungi was not found. The consistency of the lotion did not change from the original condition of the product. This research can be used as a guideline for the utilization of the extract from E. hygrophilus for commercial development accordingly.
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