Preliminary Study of Plant Extracts with Antibacterial Activity against Enteric and Skin Pathogens

Authors

  • Thien Thiraworawong Biology Program, Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University
  • Jariwat siri-in Biology Program, Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University

Keywords:

plant extracts, antibacterial activity, enteric pathogens, skin pathogens

Abstract

This research aimed to study antibacterial activity of 12 plant extracts consisting of Musa (ABB Group) Thepanom fruit, M. (ABB group) Kluai Som fruit, M. (ABB group) Kluai Hak Muk fruit, M. acuminata Colla fruit, Punica granatum L. peel, Sandoricum koetjape (Burm.f.) Merr. peel, Dimocarpus longan Lour. peel and seed, Coffea arabica L. leaf, Hevea brasiliensis Müll.Arg. leaf, Terminalia catappa L. leaf, and Psidium guajava L. leaf using 95% ethanol, 70% ethanol, and distilled water. These were examined against enteric and skin pathogens (Aeromonas hydrophila TISTR 1321, Escherichia coli TISTR 117, Salmonella Typhimurium TISTR 2519, Vibrio parahaemolyticus TISTR 1596, Pseudomonas aeruginosa TISTR 2370, Staphylococcus aureus TISTR 518, and S. aureus TISTR 2329) by disc diffusion method. The results showed that all of the pathogens were inhibited by the extracts of M. (ABB Group) “Thepanom” fruit, M. (ABB group) “Kluai Hak Muk” fruit, P. granatum L. peel, S. koetjape (Burm.f.) Merr. peel, and D. longan Lour. peel and seed. The A. hydrophila TISTR 1321 showed the largest inhibition zone by P. granatum L. peel extracted by 70% ethanol (18.3±1.6 mm). The E. coli TISTR 117 showed the largest inhibition zone by M. (ABB group) “Kluai Hak Muk” fruit extracted by 95% ethanol (21.9±1.0 mm). The S. Typhimurium TISTR 2519 showed the largest inhibition zone by M. acuminata Colla fruit extracted by 70% ethanol (15.3±0.4 mm). The V. parahaemolyticus TISTR 1596 showed the largest inhibition zone by S. koetjape (Burm.f.) Merr. peel extracted by 70% ethanol (24.1±0.6 mm). The P. aeruginosa TISTR 2370 showed the largest inhibition zone by D. longan Lour. seed extracted by water (24.9±3.2 mm). The S. aureus TISTR 518 and S. aureus TISTR 2329 showed the largest inhibition zone by M. (ABB group) “Kluai Hak Muk” fruit extracted by 95% ethanol (21.3±2.1 mm and 31.6±0.6 mm, respectively). In conclusion, these plant extracts were proven to inhibit enteric and skin pathogens.

References

Al-Mariri, A., & Safi, M. (2014). In vitro antibacterial activity of several plant extracts and oils against some gram-negative bacteria. Iranian Journal of Medical Sciences, 39(1), 36-43.

Azwanida, N. N. (2015). A review on the extraction methods use in medicinal plants, principle, strength and limitation. Medicinal & Aromatic Plants, 4(3), 1-6. doi: 10.4172/2167-0412.1000196

Boonyasiri, A., Tangkoskul, T., Seenama, C., Saiyarin, J., Tiengrim, S., & Thamlikitkul, V. (2014). Prevalence of antibiotic resistant bacteria in healthy adults, foods, food animals, and the environment in selected areas in Thailand. Pathogens and global health, 108(5), 235–245. https://doi.org/10.1179/2047773214Y.0000000148

Elisha, I. L., Botha, F. S., McGaw, L. J., & Eloff, J. N. (2017). The antibacterial activity of extracts of nine plant species with good activity against Escherichia coli against five other bacteria and cytotoxicity of extracts. BMC Complementary and Alternative Medicine, 17(1), 133. https://doi.org/10.1186/s12906-017-1645-z

Imam, M. Z., & Akter, S. (2011). Musa paradisiaca L. and Musa sapientum L.: A phytochemical and pharmacological review. Journal of Applied Pharmaceutical Science, 01(05), 14-20. https://www.japsonline.com/admin/php/uploads/78_pdf.pdf

Khamsarn, S., Nampoonsak, Y., Busamaro, S., Tangkoskul, T., Seenama, C., Rattanaumpawan, P., Boonyasiri, A., & Thamlikitkul, V. (2016). Epidemiology of antibiotic use and antimicrobial resistance in selected communities in Thailand. Journal of the Medical Association of Thailand, 99(3), 270-275. (in Thai)

Lertsethtakarn, P., Silapong, S., Sakpaisal, P., Serichantalergs, O., Ruamsap, N., Lurchachaiwong, W., Anuras, S., Platts-Mills, J. A., Liu, J., Houpt, E. R., Bodhidatta, L., Swierczewski, B. E., & Mason, C. J. (2018). Travelers' Diarrhea in Thailand: A Quantitative Analysis Using TaqMan® Array Card. Clinical infectious diseases: An official publication of the Infectious Diseases Society of America, 67(1), 120–127. https://doi.org/10.1093/cid/ciy040

Malviya, S., Arvind, Jha, A., & Hettiarachchy, N. (2014). Antioxidant and antibacterial potential of pomegranate peel extracts. Journal of Food Science and Technology, 51(12), 4132-4137. https://doi.org/10.1007/s13197-013-0956-4

Manikandan, C., & Amsath, A. (2013). Antimicrobial resistance of enteric pathogens isolated from children with acute diarrhoea in Pattukkottai, Tamil Nadu, India. International Journal of Pure and Applied Zoology, 1(2), 139-145. https://www.alliedacademies.org/articles/antimicrobial-resistance-of-enteric-pathogensisolated-from-children-with-acute-diarrhoea-inpattukkottai-tamil-nadu-india.pdf

Ministry of Public Health. (2015). Landscape of antimicrobial resistance situation and action in Thailand. Bangkok: Aksorn Graphic and Design Publishing House. (in Thai)

Mokbel, M. S. & Hashinaga, F. (2005). Antibacterial and antioxidant activities of banana (Musa, AAA cv. Cavendish) fruits peel. American Journal of Biochemistry and Biotechnology, 1(3), 125-131. https://doi.org/10.3844/ajbbsp.2005.125.131

Mostafa, A. A., Al-Askar, A. A., Almaary, K. S., Dawoud, T. M., Sholkamy, E. N., & Bakri, M. M. (2018). Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases. Saudi Journal of Biological Sciences, 25(2), 361–366. https://doi.org/10.1016/j.sjbs.2017.02.004

Phokhaphan, P., Tingpej, P., Apisarnthanarak, A., & Kondo, S. (2017). Prevalence and antibiotic susceptiblity of methicillin resistant Staphylococcus aureus, collected at Thammasat University hospital, Thailand, August 2012-July 2015. The Southeast Asian journal of tropical medicine and public health, 48(2), 351-359. https://www.tm.mahidol.ac.th/seameo/2017-48-2/09-7054-18-351-359.pdf. (in Thai)

Pornsakda, S., Plainsirichai, M., Srisaeard, K., Nakornreab, M., Laopraserd, P., Vichitsoonthonkul, T., & Photchanachai, S. (2010). Effect of Plate brush (Solanum torvum Sw.) crude extract on the inhibition of pathogenic bacteria (Escherichia coli, Staphylococcus aureus and Salmonella typhimurium). Agricultural Science Journal, 41(3/1) (Suppl.), 573-576. http://www.crdc.kmutt.ac.th/document/download/agr/agr4/573-576.pdf. (in Thai)

Pruksananonda, P., Athirakul, K., Worawattanakul, M., Varavithya, W., Pisithpun, A., Kitayaporn, D., & Anuras, S. (2008). Diarrhea among children admitted to a private tertiary-care hospital, Bangkok, Thailand: A case series. The Southeast Asian journal of tropical medicine and public health, 39(3), 434–442. https://www.tm.mahidol.ac.th/seameo/2008_39_3/09-4201.pdf

Ramakrishnan, K., Salinas, R. C., & Agudelo Higuita, N. I. (2015). Skin and soft tissue infections. American family physician, 92(6), 474-483.

Shah, M., Kathiiko, C., Wada, A., Odoyo, E., Bundi, M., Miringu, G., Guyo, S., Karama, M., & Ichinose, Y. (2016). Prevalence, seasonal variation, and antibiotic resistance pattern of enteric bacterial pathogens among hospitalized diarrheic children in suburban regions of central Kenya. Tropical Medicine and Health, 44, 39. https://doi.org/10.1186/s41182-016-0038-1

Taweewigyakarn, P., Swaddiwudhipong, W., Kanjanahong, S., Sin-anan, N., Thanakitjaroenkul, J., & Karnjanapiboonwong, A. (2014). Methicillin-susceptible Staphylococcus aureus outbreak of skin infection among neonates in a private hospital in Bangkok, 2013. Outbreak, Surveillance and Investigation Reports, 7(4), 12-18. http://www.osirjournal.net/index.php/osir/article/view/34. (in Thai)

Teague, N. S., Srijan, A., Wongstitwilairoong, B., Poramathikul, K., Champathai, T., Ruksasiri, S., Pavlin, J., & Mason, C. J. (2010). Enteric pathogen sampling of tourist restaurants in Bangkok, Thailand. Journal of travel medicine, 17(2), 118–123. https://doi.org/10.1111/j.1708-8305.2009.00388.x

Tognetti, L., Martinelli, C., Berti, S., Hercogova, J., Lotti, T., Leoncini, F., & Moretti, S. (2012). Bacterial skin and soft tissue infections: Review of the epidemiology, microbiology, aetiopathogenesis and treatment: A collaboration between dermatologists and infectivologists. Journal of the European Academy of Dermatology and Venereology: JEADV, 26(8), 931–941. https://doi.org/10.1111/j.1468-3083.2011.04416.x

Treeprasertsuk, S., Thepsuthammarat, K., Kitsahawong, B., & Phaosawasdi, K. (2016). Acute diarrhea, a significant burden to Thailand’s universal health care system: A nationwide database. Asian Biomedicine, 10(1), s23-s30. https://doi.org/10.5372/1905-7415.1000.518

Tseng, H. C., Wu, W. T., Huang, H. S., & Wu, M. C. (2014). Antimicrobial activities of various fractions of longan (Dimocarpus longan Lour. Fen Ke) seed extract. International Journal of Food Sciences and Nutrition, 65(5), 589-593. https://doi.org/10.3109/09637486.2014.886181

Visutthi, M. (2017). Antagonistic effect of staphylococci of extracts from some local plants in Nakorn-Ratchasima Province. KKU Science Journal, 45(4), 805-816. (in Thai)

Zhang, S. X., Zhou, Y. M., Xu, W., Tian, L. G., Chen, J. X., Chen, S. H., Dang, Z. S., Gu, W. P., Yin, J. W., Serrano, E., & Zhou, X. N. (2016). Impact of co-infections with enteric pathogens on children suffering from acute diarrhea in southwest China. Infectious Diseases of Poverty, 5(1), 64. https://doi.org/10.1186/s40249-016-0157-2

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Published

2020-12-09

How to Cite

Thiraworawong, T. ., & siri-in, J. (2020). Preliminary Study of Plant Extracts with Antibacterial Activity against Enteric and Skin Pathogens. EAU Heritage Journal Science and Technology (Online), 14(3), 99–114. retrieved from https://he01.tci-thaijo.org/index.php/EAUHJSci/article/view/245230

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Research Articles