Methamphetamine Analysis from Non-Extract Urine, Bile and Gastric Contents Using Paper Spray Mass Spectrometry
Keywords:
paper spray mass spectrometry, orbitrap, Methamphetamine, urine, bile, gastric contentsAbstract
Forensic toxicology laboratories typically use a two-step process to detect methamphetamine in specimen, the first step is screening such as Immunoassays method. Positive identifications during the initial amphetamines screening step are confirmed by Liquid Chromatography Mass Spectrometry--LC–MS/MS method that is the most widely used method for drug confirmation and quantitation due to its excellent sensitivity and selectivity but take a lot of time to analysis per sample. Paper spray mass spectrometry (orbitrap); PS-MS (orbitrap) is a novel method for identify drugs and substances. The aim of this study was to study the possibility of detecting methamphetamines from urine, bile and gastric contents (non-extract) using PS-MS (orbitrap) method, as a guideline for the selection of the appropriate method for identification and determination of methamphetamine. The results indicated that urine specimens are suitable for qualitative and quantitative tests due to low limit of detection and limit of detection confirmed, 1 and 250 nanograms per milliliter, respectively. This qualitative methamphetamine test in bile and gastric content samples is not suitable and has a high false negative, percent true positive rate with 3 passed parameters equal to 58.82 and 52.94, respectively. This could be solved by searching for a paper spray solvent formula that can better extract methamphetamine from the sample.
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