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The purpose of this study was to evaluate the antimicrobial activity of crude methanolic extract from the Harrisonia perforata (Blanco) Merr. species and to investigate the group of chemical compounds that have antimicrobial activity. The methanolic extracts were investigated for antimicrobial activities against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Candida albicans by disc diffusion method. The methanolic extract of Harrisonia perforata (Blanco) Merr. species exhibited antimicrobial activity against S. aureus and B. subtilis with clear zone diameter of 7.01 mm. and 6.89 mm., respectively. The H. perforata methanolic extract was selected for further investigation of active constituents by TLC-bioautography. The extract was also fragmented by Column Chromatography into two fractions, fraction 1 and 2. Fraction 1 showed antimicrobial activity against S. aureus and B. subtilis with clear zone diameter of 11.45 mm. and 8.96 mm. respectively. Fraction 2 was active against only B. subtilis with clear zone diameter of 10.77 mm. In the phytochemical constituents testing, it was found that H. perforata (Blanco) Merr. contained mainly tannins, flavonoids, alkaloids, anthraquinone glycoside, triterpenoids and steroids.
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