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The purpose of this study was to investigate the pharmacognostic specification of Baliospermum solanifolium (Burm.) Suresh leaves and roots. Thin layer chromatography (TLC) technique and Shinoda’s test were used for identify root extract. The microscopical studies of B. solanifolium leaves and roots from three provinces (Phetchaburi, Lampang and Khon Kaen) showed paracytic type of stomata, vessel, bordered pitted vessel and rosette aggregate crystals from leaves. Root powder showed spiral vessel, bordered pitted vessel, and unicellular trichome. There were some differences in appearance of cells or tissues between different sources. TLC technique showed that the solvent system (hexane: dichloromethane (2: 8), toluene : ethyl acetate (7 : 3), toluene : ethyl acetate (7 : 3), dichloromethane : methanol (6 : 4) were suitable for hexane, dichloromethane, ethyl acetate and ethanol extract respectively. The Shinoda’s test showed positive result, we expect that the roots extract have flavonoids.