Microemulsion Formulation Development of Moringa oleifera Seed Extract with Antioxidant Effects

Abstract

The objective of this experimental research was to explore the antioxidant activity of Moringa oleifera seed extract (Moringa oleifera) and. methods for preparing and developing formulations of microemulsion using Moringa seed extract.

The results of the study revealed that extract from Moringa oleifera seeds with ethyl alcohol with a concentration of 95 percent were dark brown and viscous. According to the antioxidant activity test, DPPH Assay and ABTS Assay, IC₅₀ values were 54.46±0.02 µg/ml and 26.47±0.03 µg/ml, respectively. Pseudo-ternary phase diagram was used to formulate microemulsion. The suitable microemulsion formulation consisted of Isopropyl myristate, mixed surfactant, and water 10, 50, 40 % w/w. The solution was clear yellow and non-segregated. The product formulation was developed into a microemulsion under room temperature conditions for 6 cycles under accelerated conditions, and the microemulsion was stable.

In conclusion, Moringa oleifera seed extract can inhibit free radicals, and stable microemulsions can be further developed into cosmetic products or health products.