Effects of Zinc on Peptide Profiles and Identification Score Value of Human Pathogen Vibrio cholerae Identified by MALDI-TOF MS
Keywords:
MALDI-TOF MS, Zinc, Vibrio cholerae, Identification score, BiotyperAbstract
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique was used to examine the protein patterns of bacteria and compare them with existing databases to determine their genus and species rapidly and accurately. Due to its high sensitivity, it is also capable of effectively detecting changes in protein expression. Zinc is widely used as a supplement and is included in many beverages due to its immune-enhancing, beauty-enhancing properties and used to reduce the severity of diarrhea. However, the impact of zinc on pathogen identification such as Vibrio cholerae using MALDI-TOF MS remains unclear. Therefore, this study aimed to examine the impact of zinc on the peptide mass pattern and identification score of pathogenic and non-pathogenic strains. V. cholerae was challenged with zinc concentrations below the metal tolerance value, ranging from 0.2 to 0.8 mM zinc and analyzed by MALDI-TOF MS. The analysis demonstrated a significant reduction in the identification score in relation to the concentration of zinc (p < 0.05). However, this reduction did not affect the accuracy of identifying the genus and species. These findings were consistent with the results of peptide pattern analysis by principal component analysis (PCA) in pathogenic strains but not in non-pathogenic strains (p <0.01). Furthermore, the analysis revealed distinct peptide patterns and identification score in pathogenic strains compared to control, leading to the misidentification of strains as non- pathogenic at a zinc concentration of 0.8 mM. These strains exhibited peptides specific to zinc, indicating a dose-response characteristic at peptide positions m/z 4745, 5122, 6275, and 7160 Dalton. In conclusion, this study significantly contributes to our understanding of how zinc influences the identification of pathogenic bacterial types using MALDI-TOF MS. Additionally, it provides valuable insights into potential strategies for improving the accuracy in situations where interference from zinc present in food and supplements for future investigations.
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