Compare Simple Smear method with Quantitative Formalin Ethyl Acetate Concentration Technique and Agar Plate Culture Method for Diagnosis Strongyloidiasis In feces, patients infected with HIV

Abstract

          Strongyloidiasis caused by small round worm, Strongyloides stercoralis. The disease spread worldwide, mostly occur in immunocompromise host such as malabsorbtion children, HIV infected patients, Leukemia, Lymphoma and SLE.1,2 The objective of this study was to compare method for the detection of S. stercoralis, to compare the Simple smear with the Quantitative Formalin Ethyl Acetate Concentration Technique and to compare the Agar Plate Culture with the Quantitative Formalin Ethyl Acetate Concentration Technique from stool of the 369 HIV infected patients. The sensitivity, specificity and accuracy of Simple smear were 43.3%, 100.0% and 95.4 % respectively, kappa coefficient is 0.58 (p < 0.000). That mean Simple smear has sensitivity lower than Quantitative Formalin Ethyl Acetate Concentration Technique and Agar Plate Culture. The Agar Plate Culture compared with Quantitative Formalin Ethyl Acetate Concentration Technique from the same sample, the sensitivity, specificity and accuracy were 100.0%, 93.5% and 94.0% respectively, kappa coefficient is 0.70 (p < 0.000). The detective rate of S. stercoralis by Simple smear is 3.5%, Quantitative Formalin Ethyl Acetate Concentration Technique is 8.1% and Agar Plate Culture is 14.1%. The highest detective rate of S. stercoralis is Agar Plate Culture that can enable for using as detection method.