Antibacterial Activity of Eupotorium ordortum and Centella asiatica Crude Leaf Extracts Against Staphylococcus aureus


  • Tayakee Phonlam Faculty of Medical Science, Nakhon Ratchasima College
  • Rosjarin Saeso Faculty of Medical Science, Nakhon Ratchasima College
  • Juthawadee Rittison Faculty of Medical Science, Nakhon Ratchasima College
  • Thanapat Suebrasri Faculty of Medical Science, Nakhon Ratchasima College
  • Onuma Chansatein Faculty of Education, Nakhon Ratchasima College


Antibacterial Activity, Eupotorium odortum, Centella asiatica, Crude Leaf Extracts and Staphylococcus aureus


Siam weed (Eupotorium odortum) and Asiatic pennywort (Centella Asiatica) have been used in humans and animals to treat infectious diseases, especially on their skin infection and gastrointestinal intoxication. Staphylococcus aureus is a Gram-positive bacterium. It is caused by skin and soft tissue infections. Moreover, enterotoxigenic staphylococci are the major food-borne diseases which are produced by S. aureus. The research objective was to compare E. odortum and C. Asiatica crude leaf extracts against S. aureus of Thailand Institute of Scientific and Technological Research (TISTR) 1466; (S. aureus TISTR 1466) using the paper disc diffusion method. Kanamycin 30 µg and sterile distilled water were positive and negative controlled, respectively. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were measured by the broth macrodilution method. MIC of extracted E. odortum and C. Asiatica leaves were 6.25 mg/mL. Spread plate technique on mannitol salt egg yolk (MSEY) agar was estimated for minimal bactericidal concentration (MBC) while observed of the bacterium with no growth. MBC of extracted E. odortum and C. Asiatica leaves were 25.0 and 12.5 mg/mL, respectively. Therefore, both plants could be a potential alternative for treating human and animal infectious diseases.


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How to Cite

Phonlam, T. ., Saeso, R. ., Rittison, J. ., Suebrasri, T., & Chansatein, O. (2022). Antibacterial Activity of Eupotorium ordortum and Centella asiatica Crude Leaf Extracts Against Staphylococcus aureus. EAU Heritage Journal Science and Technology, 16(1), 99–105. Retrieved from



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