A preliminary study of occurrence of ABO blood group, Rh(D) type, red blood cell antibody and antibody sensitized on red blood cell in patients without history of blood transfusion or pregnancy at Klaeng Hospital

Abstract

Pretransfusion testing consisting of ABO blood grouping, Rh(D) typing, antibody screening and crossmatch presented prevalence or occurrence of ABO blood group, Rh(D) type, red blood cell antibody, and antibody sensitized on red blood cells which differed in populations of each geographic area. This study investigated the occurrence of ABO blood group, Rh(D) type, red blood cell antibody and antibody sensitized on red blood cells in patients without history of blood transfusion or pregnancy at Klaeng Hospital. ABO blood grouping, Rh(D) typing, antibody screening, and direct antiglobulin test by conventional tube technique were conducted in a total of 81 samples. The results of the ABO blood group showed that 32 samples of O (39.50%), 26 samples of B (32.10%), 13 samples of A (16.05%), 4 samples of AB (4.94%), and 6 samples of unreported ABO blood group (7.41%). The results of Rh(D) typing presented that 80 samples of Rh(D) positive (98.77%), 1 sample of Rh(D) negative (1.23%) and weak D was not found. The antibody screening test results displayed that 79 samples of negative for antibody screening (97.53 %) and 2 samples had positive for antibody screening test (2.47%). All 81 samples showed negative direct antiglobulin test results (100.00%).    In conclusion, a preliminary study of occurrences in the first blood transfusion patients at Klaeng Hospital were as follows: group O was the most common in the ABO blood group, Rh(D) positive were 98.77%, red blood cell alloantibodies were 2.47%, similar to related studies in Thai populations.