(บทความวิจัย) การศึกษาคุณภาพผลิตภัณฑ์เกล็ดเลือดชนิดเม็ดเลือดขาวน้อยที่สุด ที่เตรียมด้วยวิธี Pooled Buffy Coat และวิธี Pooled Platelet Concentrate

Sirinya Promsorn

Authors

Sirinya Promsorn Department of Medical Technology and Clinical Pathology, Loei Hospital, Loei Province, Thailand

Keywords:

Leukodepleted pooled platelet concentrates, Quality control, Preparation time

Abstract

Platelets play a vital role in the treatment of various diseases such as dengue fever, cancer, and aplastic anemia. Modern platelet preparation technologies have advanced significantly. Among the available options, the most cost-effective and safest method for preparing platelets from multiple donors is the leukodepleted pooled platelet concentrates (LDPPC). The blood bank at Loei Hospital aimed to improve the quality of LDPPC products in alignment with international standards, ensuring the highest level of patient safety, shortening production time, reducing costs, and minimizing overtime workload. This study compared product quality between the conventional method, pooled buffy coat (P-BC), and the newer method, pooled platelet concentrates (P-PC), focusing on volume, platelet yield, leukocyte count, pH, swirling, and preparation time. This observational study utilized top-and-bottom type quadruple blood bags collected during March to May 2025. LDPPC products were randomly selected for quality inspection according to the standard sampling plan. The units were divided into two groups of 36. The data were analyzed using descriptive and inferential statistics based on the nature of each variable. Results indicated that P-PC produced a significantly greater volume than P-BC (p < 0.001) but a lower platelet yield (p = 0.006). The swirling scores for P-PC ranged from 3+ to 5+, whereas P-BC predominantly achieved 5+ (p = 0.001). P-PC also required significantly less preparation time compared to P-BC (p < 0.001). There were no significant differences in pH or leukocyte count between the two groups. In conclusion, the P-PC method met the Council of Europe (COE) standards and offered shorter preparation time. It is therefore recommended for LDPPC production to improve efficiency and reduce resource and budget burdens in clinical practice.

References

Chiewsilp P, Bejrachandra S, Thongthaisin A, editors. Standards for blood banks and transfusion services. 5th ed. Samut Prakan: Udomsuksa Publishing; 2024. (in Thai)

Aonseng S, Insawang J. Preparation of whole blood-derived platelet component. J Hematol Transfus Med 2020; 30: 379-92. (in Thai)

Lebkhurt M, Worachun N, Chanyim P, Saneesod S. Study of the quality of leukodepleted pooled platelet concentrates (LDPPC) treated with pathogen inactivation: amotosalen-UVA light treatment through 7 days of storage. J Hematol Transfus Med 2023; 33: 269-76. (in Thai)

Kanpai P, Kladed S, Siriboonrit U, Meesamut V, Permpikul P. Essential composition of blood products: what is different in platelet concentrate and pooled leukocyte-poor platelet concentrate?. J Hematol Transfus Med 2019; 29: 91-9. (in Thai)

Klom-Iam A, Witthayawiwat P. Study on the centrifugation speed that affects swirling phenomenon in platelet concentrates by using Heraeus Cryofuge 8500i. J Hematol Transfus Med 2009; 19: 87-92. (in Thai)

Chiewsilp P, Bejrachandra S, Thongthaisin A, editors. The appropriate use of blood and blood components. 2nd ed. Bangkok: Pimnapat Publishing; 2023. (in Thai)

Doerr P, editor. Guide to the preparation, use, and quality assurance of blood components. 22nd ed. Strasbourg: European Directorate for the Quality of Medicines & HealthCare (EDQM), Council of Europe; 2025. ISBN: 978-92-871-9481-7.

Bunvisuthi P, Tanthien P, Chanthnakajornfung A. Quality and stability of the inline leukocyte filter blood bag assembled by National Blood Centre, Thai Red Cross Society. Thai J Hematol Transfus Med 2002; 12: 105-13. (in Thai)

Kalanchai L, Vejbaesya S, Chongkolwatana V, Siriboonrit U, Wichitchinda K, Suratanarungsun V. Effectiveness and comparison of two filters between Sepacell R-500(II) and Sepacell RZ-200B1 in pre-storage leukocyte reduction. Thai J Hematol Transfus Med 2006; 16: 15-24. (in Thai)

El-Sayed H, Hassanein A, Fawzy R, Zahran M, Mahmoud O, Yacoub E, et al. Impact of different preparation methods on the in vitro quality of 8-day storage platelet concentrates. Life Sci J 2014; 11: 50-7.