A Comparison of Urinalysis Using Dipstick Tests and Urine Culture for Diagnosing Urinary Tract Infections at Hat Yai Hospital, Songkhla Province
Keywords:
Rapid urine strip test, Urinary tract infection, Nitrite, Leukocyte esterase, total WBCAbstract
This research is a comparative study between the rapid urine strip tests, nitrite (N) and leukocyte esterase (LE) and white blood cell count (WBC) and urine culture for their diagnostic performance. A cross-sectional analytical study was done. Urine samples were collected from patients suspected of urinary tract infections (UTIs) and examined by rapid urine strip test and urine culture between March 1st and September 30th, 2021. A total of 573 samples were included in the study. The comparison of workload and data collection costs between March 1stand September 30th, 2021. It was found that microorganisms were identified by urine culture in 49.91% of the samples, predominantly Gram-negative bacteria. The most common organism was Escherichia coli, followed by Klebsiella pneumoniae and Enterococcus faecalis at 53.67%, 10.26%, and 7.04%, respectively. The combination of dipstick tests N, LE, and WBC demonstrated sensitivity, specificity, PPV, NPV, and accuracy values of 92.66%, 43.55%, 62.06%, 85.62%, and 68.06%, respectively. The positive and negative likelihood ratios were 1.64 and 0.17, respectively. This approach can be an effective screening tool for UTIs before conducting urine culture. The implementation of initial screening using dipstick tests performed by ward nurses, followed by secondary screening in the laboratory, resulted in a reduction in both workload and costs.References
Permpongkosol S. Urinary Tract Infections [Internet]. 2018 [cited 2025 May 3]. Available from:
http://www.rama.mahidol.ac.th/surgery/sites/default /files/public/pdf/Urinary%20tract%20infection.pdf 2. Khazaei S, Ayubi E. Importance of prevention and control of nosocomial infections in Iran. Iran J Public Health 2018; 47: 307-8. 3. Cheechang S. Trends in Incidence and Drug-Resistant on Bacterial Infection Among Patients in Sichon Hospital, Nakhon Si Thammasat Province. R3 Med PHJ 2024; 21: 23-30. (in Thai)
He Y, Zhao J, Wang L, et al. Epidemiological trends and predictions of urinary tract infections in the global burden of disease study 2021. Sci Rep 2025; 15: 1-12.
Bendall MJ. A review of urinary tract infection in the elderly. J Antimicrobe Chemother1984; 13: 69–78.
Chang R, Green MT, Chenoweth CE, et al. Epidemiology of hospital-acquired urinary tract-related bloodstream infection at a university hospital. Infect Control Hosp Epidemiol 2011; 32: 1127-9.
National Institute of Health, Department of Medical Sciences, Ministry of Public Health. Manual for the operation of bacteria and fungi for general and general hospitals. 3rd ed. Bangkok: Premier Marketing Solutions Company Limited; 2018. (in Thai)
Schnarr J, Smaill F. Asymptomatic bacteriuria, and symptomatic urinary tract infections in pregnancy. Eur J Clin Invest 2008; 38: 50-7.
Mancuso G, Midiri A, Gerace E, Marra M, Zummo S, Biondo C. Urinary Tract Infections: The Current Scenario and Future Prospects. Pathogens 2023; 12: 1-17.
Wikipedia. Urine test strip [Internet]. 2025 [cited 2025 May 3]. Available from:
http://en.wikipedia.org/wiki/Urine_test_strip
Chupunya K. Urine Examination Manual,. Textbook. Project - Siriraj Faculty of Medicine Siriraj Hospital Mahidol University, Bangkok, 1985. (in Thai)
Waterfield T, Foster S, Platt R, et al. Diagnostic test accuracy of dipstick urinalysis for diagnosing urinary tract infection in febrile infants attending the emergency department. Arch Dis Child 2022; 107: 1095–9.
Infectious Disease Association of Thailand. Clinical Practice Guidelines for the Treatment of Urinary Tract Infections [Internet]. 2025 [cited 2025 May 3]. Available from: http://www.cimjournal.com/nephro-article/guideline-2/2568
Helan S, Lee JL. Urinary Tract Infection. Pharmacotherapy Self-Assessment Program (PSAP), 2018 Book 1 Infection Diseases 2018; 3: 7-30.
Preechasuchakul B, Sukopokvech A. Urinalysis and body fluids. Bangkok: Department of Clinical Microscopy. Faculty of Medical Technology Mahidol University; 2000. (in Thai)
Deville WL, Yzermans JC, van Dujin NP, Bezemer PD, van der Windt DA, Bouter LM. The urine dipstick test useful to rule out infections. A meta-analysis of the accuracy. BMC Urology 2004; 4: 1-14.
Ralukruedej S. Evaluation of urine strip test for the diagnosis of urine trace infection at Police General hospital. Chiang Mai Assoc Med Sci. 2009; 44: 106-9. (in Thai)
Marques AG. Doi AM, Pasternak J. Performance of the dipstick screening test as a predictor of negative urine culture. Einstein. 2017; 15: 34-9.
Fernandes DJ, Devdas JM, Castelino DN. Utility of dipstick test (nitrite and leukocyte esterase) and microscopic analysis of urine when compared to culture in the diagnosis of urinary tract infections in children. Int J Contempt Podiatry 2018 ; 5: 156-60.
Pongsevee M. Urine and Body Fluid Analysis 2nd. Bangkok: Thammasat University Press; 2014. (in Thai)
Lebnark T. Urinalysis and body fluids. Bangkok: Samcharoen Panitch (Bangkok) Co., Ltd; 1991. (in Thai)
Gallagher EJ, Schwartz E, Weinstein RS. Performance characteristics of urine dipsticks stored in open container. Am J Emerge Med. 1990; 8: 121-3.
Chonsawat P, Wongphan B, Satayarak J. Comparison of Urine dipstick and Urine culture in Diagnosis of Urinary tract Infection. J Med Tech Thailand 2020; 48: 7548-59. (in Thai)
Hantalom R, Sriprom K, Kawinpanitan S. The accuracy of leukocyte esterase urine strip for diagnosis of peritoneal dialysis. Chiangrai Med J 2023: 15: 119-28. (in Thai)
Sutthiphan K, Noppamas K. Atlas of Urinary. 2nd ed. Khonkaen: Klungnanawittaya Printing Limited Partnership; 2018. (in Thai)
Masoud SS, Majigo M, Silago V, Kunambi P, Nyawale H, Moremi N. Utility of dipstick urinalysis in the diagnosis of urinary tract infections among outpatients in Mwanza and Dar es Salaam regions in Tanzania. Bull Natl Res Cent 2024; 48: 1-7.
Parveen R, Rahim I. Study of bacterial pathogens in urinary tract infection and their antimicrobial sensitivity pattern. Bangladesh J Infect Dis 2017; 4: 40-4.
Kristensen LH, Winther R, Colding-Jørgensen JV, Pottegård A, Nielsen H, Bodilsen J. Diagnostic accuracy of dipsticks for urinary tract infections in acutely hospitalized patients: a prospective population-based observational cohort study. BMJ Evid Based Med 2025; 30: 36-44.
Pongpipatpaiboon K, Wattanapan P, Arayawichaon P. The Use Dipstick for Screening of Urinary Tract infection in Patients with Neurogenic Bladder Secondary to Spinal Cord Lesion. J Thai Rehabil Med 2013; 23(1): 1-6. (in Thai)
Hunt KM, MD, Green RS, Sartori LF, et al. Urine Dipstick for the Diagnosis of Urinary Tract Infection in Febrile Infants Aged 2 to 6 Months. PEDIATRICS 2025; 155: 1-7.
Patel HD, Livesey SA, Swann RA, Bukhari SS. Can urine dipstick testing for urinary tract infection at point of care reduce laboratory workload? Clin Patrol 2005; 58: 951- 4.
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