Identification of Key RNA Target from Human Neutrophils Associated with Salmonella Infection
Keywords:
Neutrophils, Salmonella, Diacylglycerol O-acyltransferase 2, DGAT2Abstract
Salmonella enterica serovar Typhimurium (S. Typhimurium) typically causes food-borne gastroenteritis which is a major impact on global public health issues. The pathological hallmark of S. Typhimurium-induced enterocolitis in humans is associated with a massive neutrophil influx into the intestine. Uncontrolled activation and infiltration of neutrophils lead to dysregulation of inflammatory responses and tissue damage. Currently, numerous studies in the field of transcriptomics have been conducted, leading to a growing database of research output and resource for transcriptomic analyses. The aim of this study was identification of key RNA target from human neutrophils associated with Salmonella infection among an RNA collection of public transcriptome database. The obtained data was subsequently processed with simple arrangement of significant neutrophil genes. The results demonstrated that diacylglycerol O-acyltransferase 2 (DGAT2) was selected among 137 significant neutrophil genes relevant to Salmonella infection compared to healthy controls. Moreover, this target also highly rated for its novelty which has never been studied in relation to Salmonella or neutrophils. Thus, the selection of this novel gene was suitable to be applied for further study. To investigate a role of neutrophil DGAT2 in bacterial killing in vitro study, human neutrophils isolated from healthy subjects were infected with S. Typhimurium in the presence or absence of DGAT2 inhibitor and intracellular survival of bacteria was counted. The results indicated that the numbers of intracellular bacteria were significantly higher in the presence of DGAT2 inhibitor (177.17 ± 46.52 CFU/ml) than in the absence of DGAT2 inhibitor (77.33 ± 23.72 CFU/ml) at 60 minutes post-incubation, suggesting that targeted DGAT2 plays an important role in improving efficiency of neutrophil-mediated killing. This research utilizes and extracts various collected data, which can be applied to in vitro experiments for further studies to create valuable new knowledge related to the pathogenesis of infectious diseases.
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