Dose-Response Curves for Gamma-Ray-Induced Dicentric Chromosomal Aberrations for Radiological Emergency Preparedness in Thailand
Keywords:
Radiological emergency preparedness, Radiation biodosimetry, Dicentric chromosomal aberration, Dose-response curves, Radiological incidentsAbstract
Accurate assessment of ionizing radiation exposure is essential in the realm of biological dosimetry, particularly in regions where unique environmental and demographic factors may influence radiation responses. This study presents the development of dose-response curves for gamma-ray-induced dicentric chromosomal aberrations in human lymphocytes, intending to enhance radiological emergency preparedness in Thailand. Blood samples from a 39-year-old male and a 32-year-old female were irradiated with Co-60 gamma rays at a dose rate of 0.574 Gy/min across a dose range of 0–5 Gy. The lymphocytes were cultured and analyzed for dicentric chromosomal aberrations following standard procedures recommended by the International Atomic Energy Agency (IAEA). The dose-response curves were generated using a linear-quadratic model with Biodose Tools v3.6.1 software. Accuracy assessment of the generated curves, for both individual and pooled datasets, showed that the estimated radiation doses closely aligned with the actual delivered doses. Statistical analysis using paired t-test and ANOVA revealed no significant difference between the estimated doses from the individual and pooled curves. These findings strengthen Thailand’s capability to assess radiation exposure in emergency situations and support global efforts in biological dosimetry by establishing a reliable, locally generated reference curve.
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