Benjakul water extraction prevents the aging of kidney cells in high-fat diet-induced obese rats
Keywords:
Aging, belly obesity, Benjakul water extraction, SA-β-gal, KidneyAbstract
Study on the effects of Benjakul extract in preventing kidney cell aging in 18 male Sprague-Dawley obese rats, divided into 6 groups: a control group receiving a normal diet, a high-fat diet group, a high-fat diet group supplemented with a low-dose water extract of Benjakul, a high-fat diet group supplemented with a high-dose water extract of Benjakul, a group receiving a high-fat diet with wild betel leaf water extract, and a group receiving a high-fat diet with metformin. After a 4-week feeding period, the rats were assessed for body weight, blood sugar, insulin, blood urea nitrogen (BUN), creatinine, and senescence-associated β-galactosidase (SA-β-gal) in the kidneys. It was observed that the aqueous extract of Benjakul showed a tendency to reduce the body weight of rat starting from week 1, and it demonstrated the most significant weight reduction in weeks 3 and 4 when compared to the group receiving a high-fat diet. High-dose Benjakul water extract showed the highest insulin sensitivity, while low-dose Benjakul water extract and wild betel leaf water extract had a tendency to reduce BUN levels, indicating potential renal protection. In contrast, high-dose Benjakul water extract and metformin showed a trend towards increased BUN levels, suggesting potential kidney damage. Both low-dose and high-dose Benjakul water extracts demonstrated the ability to reduce the density of SA-β-gal staining in kidney cells, indicating a potential anti-aging effect on kidney cells in rat receiving a high-fat diet.
References
Hong C, Sun L, Liu G, Guan B, Li C, Luo Y. Response of global health towards the challenges presented by population aging. China CDC Wkly 2023; 5: 884-7.
von Frankenberg AD, Marina A, Song X, Callahan HS, Kratz M, Utzschneider KM. A high-fat, high-saturated fat diet decreases insulin sensitivity without changing intra-abdominal fat in weight-stable overweight and obese adults. Eur J Nutr 2017; 56: 431-443.
Jitnarin N, Kosulwat V, Rojroongwasinkul N, Boonpraderm A, Haddock CK, Poston WS. Prevalence of overweight and obesity in Thai population: results of the National Thai Food Consumption Survey. Eat Weight Disord 2011; 16: e242-e9.
Arner P. The adipocyte in insulin resistance: key molecules and the impact of the thiazolidinediones. Trends Endocrinol Metab 2003; 14: 137-45.
Haslam DW, James WP. Obesity. Lancet 2005; 366: 1197-209.
Barness LA, Opitz JM, Gilbert-Barness E. Obesity: genetic, molecular, and environmental aspects. Am J Med Genet A 2007; 143A: 3016-34.
Dröge W. Free radicals in the physiological control of cell function. Physiol Rev 2002; 82: 47-95.
Buttke TM, Sandstrom PA. Oxidative stress as a mediator of apoptosis. Immunol Today 1994; 15: 7-10.
Fabbrini E, Magkos F. Hepatic steatosis as a marker of metabolic dysfunction. Nutrients 2015; 7: 4995-5019.
Bürkle A, Moreno-Villanueva M, Bernhard J, et al. MARK-AGE biomarkers of ageing. Mech Ageing Dev 2015; 151: 2-12.
Hayflick L, Moorhead PS. The serial cultivation of human diploid cell strains. Exp Cell Res 1961; 25: 585-621.
López-Otín C, Blasco MA, Partridge L, Serrano M, Kroemer G. The hallmarks of aging. Cell 2013; 153: 1194-1217.
Dimri GP, Lee X, Basile G, Acosta M, Scott G, Roskelley C, et al. A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc Natl Acad Sci USA 1995; 92: 9363-7.
Li X, Zheng S, Wu G. Amino acid metabolism in the kidneys: nutritional and physiological significance. Adv Exp Med Biol 2020; 1265: 71-95.
Radabsri Y. The Development of the quality of life of the elderly in accordance with the Buddhist principles. Vanam_434 2021; 8, 109-22.
Itharat A, Sakpakdeejaroen I. Determination of cytotoxic compounds of Thai traditional medicine called Benjakul using HPLC. J Med Assoc Thai 2010; 93: S198-S203.
Rattarom R, Sakpakdeejaroen I, Hansakul P, Itharat A. Cytotoxic activity against small cell lung cancer cell line and chromatographic fingerprinting of six isolated compounds from the ethanolic extract of Benjakul. J Med Assoc Thai 2014; 97: S70-S5.
Kim KJ, Lee MS, Jo K, Hwang JK. Piperidine alkaloids from Piper retrofractum Vahl. protect against high-fat diet-induced obesity by regulating lipid metabolism and activating AMP-activated protein kinase. Biochem Biophys Res Commun 2011; 411: 219-25.
Vongthoung K, Kamchansuppasin A, Temrangsee P, Munkong N, Kaendee N, Lerdvuthisopon N. Effects of Benjakul water extract on pancreas in high-fat fed rats. TMJ 2016; 16: 161-75.
Eu CH, Lim WY, Ton SH, bin Abdul Kadir K. Glycyrrhizic acid improved lipoprotein lipase expression, insulin sensitivity, serum lipid and lipid deposition in high-fat diet-induced obese rats. Lipids Health Dis 2010; 9: 81.
Kamchansuppasin A, Vongthoung K, Temrangsee P, Munkong N, Lerdvuthisopon N. Benjakul supplementation improves hepatic fat metabolism in high-fat diet-induced obese rats. Trop J Pharm Res 2020; 19: 797-803.
Kovesdy CP. Epidemiology of chronic kidney disease: an update 2022. Kidney Int Suppl (2011) 2022; 12: 7-11.
Chantor P, Rattanabanjerdkul H, Itharat A. Case studies of Benjakul recipes in treating stage 4 non – small cell lung cancer. Thammasat Med J 2017; 16: 172-81. (in Thai)
Temrangsee P, Itharat A, Sattaponpan C, Pipatratanaseree W. Inhibitory effect on alpha-glucosidase activity of Benjakul, Soros Benjakul and their plant component. Thammasat Med J 2019; 19: 645-53. (in Thai)
Wang M, Wang Z, Chen Y, Dong Y. Kidney damage caused by obesity and its feasible treatment drugs. Int J Mol Sci 2022; 23: 747.
Raumcharoen F. Metformin-induced metabolic acidosis in diabetic patients attending Nakhon Phanom hospital, 2010-2013. J Health Sci 2015; 24: 337-46. (in Thai)
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