Incidence of carbapenemase production in Pseudomonas aeruginosa isolated from patients of Bamrasnaradura Infectious Diseases Institute
Keywords:
Pseudomonas aeruginosa, carbapenemase genes, Carba NPAbstract
Carbapenemase producing Pseudomonas aeruginosa (CPPA) is one of important causative agents of nosocomial infections and are associated with increase severity of the disease and prolong of hospitalization. The increasing carbapenem resistance of P. aeruginosa which have a broad spectrum of antibacterial activity and are used as last resort drugs for the treatment of infections. The rapid detection technique is important to provide early information for appropriate treatment. The aim of this study was to differentiate carbapenemase producing P. aeruginosa (CPPA) from carbapenemase non-producing (CNPPA) isolates by CarbaNP assay. Methods: A total of 157 non-duplicated clinical P. aeruginosa isolates were composed of 82 carbapenem resistant P. aeruginosa (CRPA) and 75 CSPA isolates. All isolates were detected CRPA with imipenem and meropenem by modified Kirby-Bauer disk diffusion method and detected CPPA by CarbaNP assay. All 82 CRPA isolates were investigated carbapenemase gene by PCR for blaIMP, blaVIM and blaNDM. For the results, all CSPA isolates were susceptible to imipenem and meropenem whereas CRPA isolates were resistant to imipenem (95.1 %) and meropenem (97.6%). Of 82 CRPA isolates, 42 (51.2%) isolates produced carbapenemase genes and 43 (52.4%) isolates were CPPA by CarbaNP assay. Almost CPPA isolates could detect within 5 minutes. However, 1 NDM producing isolate gave negative result by CarbaNP within 2 hours. In conclusion, 44 (53.6%) isolates were CPPA and the CarbaNP assay was a rapid and reliable method to differentiate CPPA from CNPPA.
References
Phumart P, Phodha P, Thamlikitkul V, Riewpaiboon A, Prakongsai P, Limwat- tananon S. Health and economic impacts of antimicrobial resistant infections in Thailand: a preliminary study. J Health Syst Res 2012; 6: 352-60.
Bocharova Y, Savinova T, Lazareva A, et al. Genotypes, carbapenemase carriage, integron diversity and oprD alterations among carbapenem-resistant Pseudomonas aeruginosa from Russia. Int J Antimicrob Agents 2020; 55: 105899. doi:10.1016/j. ijantimicag.2020.105899
Feucherolles M, Cauchie HM, Penny C. MALDI-TOF mass spectrometry and specific biomarkers: potential new key for swift identification of antimicrobial resistance in foodborne pathogens. Microorganisms. 2019; 7: 593. doi:10.3390/microorganisms7120593
Wayne PA, Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Disk Susceptibility Tests. CLSI M02, 13th ed. Clinical and Laboratory Standards Institute 2018.
Wayne PA, Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing. CLSI M100, 32th ed. Clinical and Laboratory Standards Institute 2022.
Ellington MJ, Kistler J, Livermore DM, Woodford N. Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases. J Antimicrob Chemother 2007; 59: 321-2.
Wang TH, Leu YS, Wang NY, Liu CP, Yan TR. Prevalence of different carbapen- emase genes among carbapenem- resistant Acinetobacter baumannii blood isolates in Taiwan. Antimicrob Resist Infect Control 2018; 7: 123. doi:10.1186/ s13756-018-0410-5
Bassetti M, Vena A, Croxatto A, Righi E, Guery B. How to manage Pseudomonas aeruginosa infections. Drugs Context 2018; 7: 1-18.
Biondo C. Bacterial antibiotic resistance: the most critical pathogens. Pathogens 2023; 12: 116. doi:10.3390/pathogens 12010116
El-Far A, Samir S, El-Gebaly E, et al. High rates of aminoglycoside methyltrans- ferases associated with metallo-beta- lactamases in multidrug-resistant and extensively drug-resistant Pseudomonas aeruginosa clinical isolates from a tertiary Care hospital in Egypt. Infect Drug Resist 2021; 14: 4849-58.
Tran HA, Vu TNB, Trinh ST, et al. Resistance mechanisms and genetic relatedness among carbapenem-resistant Pseudomonas aeruginosa isolates from three major hospitals in Hanoi, Vietnam (2011-15). JAC Antimicrob Resist 2021; 3: dlab103. doi:10.1093/jacamr/dlab103
Saengsuwan P, Kositpantawong N, Kawila S, Patugkaro W, Romyasamit C. Prevalence of carbapenemase genes among multidrug-resistant Pseudomonas aeruginosa isolates from tertiary care centers in Southern Thailand. Saudi Med J 2022 Sep; 43: 991-9.
Chong PM, McCorrister SJ, Unger MS, Boyd DA, Mulvey MR, Westmacott GR. MALDI-TOF MS detection of carbapenemase activity in clinical isolates of Enterobacteriaceae spp., Pseudomonas aeruginosa, and Acinetobacter baumannii compared against the Carba-NP assay. J Microbiol Methods 2015; 111: 21-3.
Bouslah Z. Carba NP test for the detection of carbapenemase-producing Pseudomonas aeruginosa. Med Mal Infect 2020; 50: 466-79.
Bernabeu S, Dortet L, Naas Thierry. Evaluation of the β-CARBATM test, a colorimetric test for the rapid detection of carbapenemase activity in Gram-negative bacilli, J Antimicrobial Chemotherapy 2017; 72: 1646-58.
Ahmad N, Ali SM, Khan AU. Detection of New Delhi Metallo-β-Lactamase Variants NDM-4, NDM-5, and NDM-7 in Enterobacter aerogenes Isolated from a Neonatal Intensive Care Unit of a North India Hospital: A First Report. Microb Drug Resist 2018; 24: 161-5.
Lee LY, Korman TM, Graham M. Rapid time to results and high sensitivity of the CarbaNP test on early cultures. J Clin Microbiol 2014; 52: 4023-6.
Osei Sekyere J, Govinden U, Essack S. Comparison of existing phenotypic and genotypic tests for the detection of NDM and GES carbapenemase-producing Enterobacteriaceae. J Pure Appl Microbiol 2016; 10: 2585-91.
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