Association of CXCL10 G-201A/C-1596T Polymorphisms with Hepatitis B and C Infection in Human Immunodeficiency Virus-infected Thais

Abstract

Previous studies demonstrated the high prevalence of hepatitis B (HBV) and C (HCV) virus coinfection in human immunodeficiency virus (HIV)-infected Thais and their genetic risk factors are still unclear. The C-X-C motif chemokine 10 (CXCL10) has been well characterized as a key regulator of viral hepatitis and the regulatory G-201A and C-1596T single nucleotide polymorphisms (SNPs) in CXCL10 gene has been demonstrated to affect the production levels of CXCL10. The present study aimed to investigate allelic and genotypic frequencies of the G-201A and C-1596T SNPs and their effect on the susceptibility of HBV and HCV infection in HIV-infected Thais. A cross-sectional study was conducted in 200 Thai HIV patients. Prevalence of HBV/HIV, HCV/HIV and HBV/HCV/HIV infection was 9.0%, 9.0% and 0.6% respectively, and rate of transaminitis was 29%. DNA samples were genotyped by a polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and confirmed by DNA sequencing. Our data demonstrated that the G-201A and C-1596T SNPs were in absolute linkage disequilibrium. Frequencies of AA/TT, GA/CT and GG/CC genotypes were 0.010, 0.225 and 0.765, and A/T and G/C alleles were 0.122 and 0.878, respectively. The Chi-square analysis indicated no significant association of the SNP genotypes and alleles with HBV and HCV infection, and CD4+ cell count in this patient group (p > 0.05). The data generated in this study support understanding in molecular genetic mechanisms for liver disease in HIV infection.