Comparative Study of Chronic Kidney Disease Stages by GFR Derived from CKD-EPI Formula Using Enzymatic and Jaffe’s Kinetic Methods in Diabetic Patients Type 2

Abstract

The  purpose  of  this  study  was  to  compare  the  eGFR  with  CKD-EPI  from  serum creatinine determined by Jaffe’s kinetic method (CrJaf) and enzymatic method (Crenz). The study

was performed in 107  type 2 diabetic patients, 49  males and 58 females with mean age of

64 years recruited from diabetic clinic at Thungsong  Hospital, Nakhon Si Thammarat Province. Highly correlated of Crenz and CrJaf in all subjects was found (r = 0.936,  p < 0.001).  However, Crenz was significantly lower compared to CrJaf (1.31  ± 0.90 mg/dL and 1.41  ± 0.87 mg/dL,

respectively, p < 0.001). Crenz   in subjects with eGFR   ≥ 60 mL/min/1.73  m2  (n = 58) was lower than CrJaf significantly (0.83  ± 0.20 mg/dL and 0.97  ± 0.22 mg/dL, respectively, p < 0.001), whereas the difference between Crenz  and CrJaf was not found in subjects with eGFR < 60 mL/ min/1.73 m2 (n = 49), (1.90  ± 1.50 mg/dL and 1.94  ± 1.05 mg/dL, respectively, p = 0.117).

eGFR-Crenz  was significantly higher than eGFR-CrJaf  in all samples (65  ± 30 and 58 ± 25 mL/

min/1.73 m2, respectively, p < 0.001)  and those with eGFR   ≥ 60 mL/min/1.73 m2  (89  ± 16 and

77  ± 15 mL/min/1.73  m2, respectively,  p < 0.001).  The number of subjects with eGFR-Crenz   and eGFR-CrJaf  ≥ 60 mL/min/1.73  m2  were  59  and 51,  and those with eGFR-Crenz  and eGFR-CrJaf 

< 60 mL/min/1.73  m2  were 48 and 56, respectively. The same number of subjects with stage 4 and stage 5 CKD of eGFR-Crenz  and eGFR-CrJaf  were 11 and 5, respectively. Therefore, using eGFR to differentiate CKD stages with creatinine determined by enzymatic method may not be superior to those with Jaffe’s kinetic method in type 2 diabetic patients.