Real Time Monitoring Cell Proliferation of SW620 Cell Line Following Co-culture with PHA-Stimulated Lymphocytes and CD44 Expression Following Cytotoxic and Apoptotic Induction

Abstract

CD44 is a cell surface protein detected in cancer stem cells of colon and other cancer types. This study aimed to real-time monitor cell proliferation by using cell index analysis of SW620 cell line following co-culture with PHA-stimulated lymphocytes. The effects of anti-CD44 antibody on cell proliferation and CD44 expression following cytotoxic and apoptotic induction were also assessed. The cell proliferation of SW620 cells was decreased by anti-CD44 antibody in dose-dependent manner and more pronounced following co-culture with PHA-stimulated lymphocytes, compared to the untreated SW620 control cells. The expression of CD44 on SW620 cell line was significantly increased after induction of cytotoxicity and apoptosis as compared to the SW620 control cells (P < 0.05, paired t-test). Our study demonstrated a role of CD44 on cell proliferation and survival of SW620 cell line. These findings may lead to further applicative study of CD44 and other specific surface markers on colorectal cancer stem cells.