การศึกษาฤทธิ์การเพิ่มจำนวนและการเคลื่อนที่ของเซลล์ของสารสกัดสมุนไพร ในเซลล์เคอราติโนไซท์

Abstract

Several Thai herbs possess wound healings properties. Wound healing process consists of many mechanisms, including stimulation of keratinocyte, collagen production, inflammation, antioxidants and wound closing by induce cell migration. This experimental study aimed to investigate in vitro cell proliferation and cell migration activities of medicinal herb extracts which related to wound healing processes in human keratinocyte cell (HaCaT). Regarding the experimental method, the medicinal herb extracts were prepared from fresh extracts, including Croton stellatopilosus,  Morus alba, Polygonum odoratum, Alternanthera sessilis and Portulaca oleracea. Activity assays were then performed in human keratinocyte covered stimulation of proliferation by MTT assay and stimulation of cell migration by the scratch assay.  The results revealed that all herbs, except Polygonum odoratum, can stimulate proliferation of HaCaT and differ from the control group significantly (p<0.05). Portulaca oleracea extract, at concentration of 10 µg/mL, can increase cell proliferation and lead to wound closure 90.29+8.15% at 72 hours which was better than untreated control (60.01+19.59%). It could be concluded that Portulaca oleracea extract can possess wound healing properties by stimulation of cell proliferation and cell migration. Mechanism of action of Portulaca oleracea should be more for further investigation in order to find supporting data for improving health products related to wound healing or applying the knowledge to other related works.   Keywords: Cell Proliferation, Cell migration, Keratinocyte cell