สารสกัดว่านชักมดลูกที่มีฤทธิ์ต้านอนุมูลอิสระและเป็นพิษต่อเซลล์มะเร็งคอมดลูก
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เผยแพร่แล้ว:
พ.ค. 15, 2019
คำสำคัญ:
ว่านชัดมดลูก สารต้านอนุมูลอิสระ
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บทคัดย่อ
The powerful antioxidative and HeLa cytotoxic activity of crude ethanol extract of Curcuma comosa Roxb. prompted us to carry out biologically-guided separation of the active constituent. Chromatographic separation of the ethanol extract yielded fractions Zcc(R)-E-1 and Zcc(R)-E-2. The potent fraction Zcc(R)-E- 1 was subsequently bioassay-guided separated and the active fraction Zcc(R)-E-15 was obtained. Recrystallization of fraction Zcc(R)-E-15 yielded compound A, exhibited antioxidant power 1064.73 ± 60.96 μM vitamin C equivalence and HeLa cell cytotoxicity with IC50 4.44 ± 0.85 μg/ml. With structure elucidation via spectroscopic techniques, compound A was identified as 4,6-dihydroxy-2-O-(β-D-glucopyranosyl)acetophenone.
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Original Articles
เอกสารอ้างอิง
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2. Piyachaturawat P, Chai-ngam N, Chuncharunee A, Komaratat P,Suksamrarn A. Choleretic activity of phloracetophenone in rats:
structure-function studies using acetophenone analogues. European J Pharmacol 2000;387:221-7.
3. Jurgens TM, Frazier EG, Schaeffer JM, Jones TE, Zink DL, Borris RP,et al. Novel nematocidal agents from Curcuma comosa. J Nat Prod 1994;57:230-5.
4. Piyachaturawat P, Ercharuporn S, Suksamrarn A. Uterotropic effect of Curcuma comosa in rats. Int J Pharmacog 1995:33(4):334-8.
5. Piyachaturawat P, Teeratagolpisal N, Toskulkao C, Suksamrarn A.Hypolipidemic effect of Curcuma comosa in mice. Artery 1997;22(5):233-41.
6. Suksamrarn A, Eiamong S, Piyachaturawat P, Byrnre TL. A phloracetophenone glucoside with choleretic activity from Curcuma comosa. Phytochemistry 1997;45:103-5.
7. Piyachaturawat P, Charoenpiboonsin J, Toskulkao C, Suksamrarn A. Reduction of plasma cholesterol by Curcuma comosa extract in hypercholesterolaemic hamster. J Ethnopharmacol 1997;66:199-204.
8. Piyachaturawat P, Timinkul A, Suksamrarn A. Effect of Curcuma comosa extract on male fertility in rats. Pharmaceutic Biol 1999;37:22-27.
9. Schumann K. Zingiberaceae. In: Engler A, editor. Berlin : Pflanzenreich;1903: IV. P. 46.
10. Backer CA, Bakhuizen van den Brink RC. Flora of Java. The Netherlands,I.N.V.P.: Noordhoff-Groningen; 1963.
11. Styhl E. Thin-layer chromatography. In: A laboratory handbook. Berlin,Heidelburg, New York: Springer-Verlag; 1969.
12. Benzie I, Strain J. Ferric reducing /antioxidant power assay. Direct measure of total antioxidant activity of biological fluids and modified
version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol 1999;299:15-27.
13. Takahashi GW, Montgomery RB, Stahl WL, Crittenden CA, Valentine MA, Thorning DR, et al. Pentoxifylline inhibits tumor necrosis factoralpha-mediated cytotoxicity and cytostasis in L929 murine fibrosarcoma cells. Int J Immunopharmacol 1994;16:723-36.
