การพัฒนาวิธีวิเคราะห์และการตรวจสอบความถูกต้องของวิธีวิเคราะห์หาปริมาณแคนนาบินอยด์ในสารสกัดกัญชาด้วยวิธีโครมาโทกราฟีของเหลวประสิทธิภาพสูง
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Abstract
Introduction and Objective: Cannabis (Cannabis sativa L.) has been used for medicinal purposes since ancient time. Tetrahydrocannabinol (THC) is the major psychoactive compound and the other non-psychoactive ingredients are cannabidiol (CBD). Cannabis has been used as an active ingredient in various pharmaceutical and health-related products. However, the appropriate analytical methods for quality control of active compounds in cannabis extracts remain limited. This study aimed to develop and validate an Ultra High Performance Liquid Chromatography (UHPLC) method for the quantification of cannabinoids in cannabis extract samples.
Methods: The study was divided into three steps: (1) developing the UHPLC method for analyzing cannabidiol and tetrahydrocannabinol in cannabis extracts, (2) testing the validity of the analysis method, and (3) analyzing the quantity of cannabidiol and tetrahydrocannabinol in 10 cannabis extract samples.
Results: This method was validated by establishing the linearity for cannabidiol and tetrahydrocannabinol at the concentration range of 24.23-290.70 and 23.98-383.62 μg/mL, respectively, with a determination correlation coefficient (r) of 0.999. The % recovery was in the range of 98.99-100.47%. The limit of detection of cannabidiol and tetrahydrocannabinol was 1.015 and 2.135% w/w (calculated on the weight as C. sativa extracts, µg/µg), respectively, while the limit of quantification were 3.382 and 7.118% w/w (calculated on the weight as C. sativa extracts, µg/µg), respectively.
Discussion: The method developed for analyzing cannabidiol and tetrahydrocannabinol in cannabis extracts using UHPLC. The validity test result for the analysis method all fell within acceptable limits. This developed analysis method requires less time for analysis and involves a simple, convenient and rapid preparation of the mobile phase.
Conclusion and Recommendation: This recently developed method proves suitable for routine quantitative analysis of cannabidiol and tetrahydrocannabinol in cannabis extracts. It can support the establishment of quality specifications for cannabis extracts.
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