Antioxidant, Anti-inflammation and Stability Properties of Ya Thappayathikhun Formula Extract

Abstract

Introduction and objectives: Ya Thappayathikhun  is a Thai traditional medicinal (TTM) formula commonly used to treat numbness due to diabetes. This research aimed to investigate the pharmacological properties of the TTM formula especially its antioxidant and anti-inflammatory activities as well as extract stability. The researchers sought to prove the pharmacological properties of this medicinal formula since no previous pharmacological studies had been conducted on it. Additionally, they aimed to confirm that the medicinal formula possesses antioxidant and anti-inflammatory properties, which can help slow down cellular deterioration, aligning with the traditional use of this formula to alleviate symptoms such as muscle pain, chest pain, numbness, loss of taste, and insomnia.

Methods: This research was conducted in a laboratory setting and involved the study of antioxidant properties using the DPPH assay, anti-inflammatory properties using the Nitric Oxide method, and an examination of the formula's stability with Piperine as the main compound, assessed through High-Performance Liquid Chromatography (HPLC) at temperatures of 50°C, 60°C, 70°C, and 80°C over durations of 3, 6, 9, and 12 days.

Results: Antioxidant properties were studied using the DPPH assay at concentrations of 5, 2.5, 1.25, 0.625, 0.312, and 0.156 mg/mL, at a light wavelength of 515 nm. The formula exhibited antioxidant properties with an effective concentration of 0.8859 mg/mL. Anti-inflammatory properties were studied using the Nitric Oxide inhibition method at concentrations of 1, 0.5, 0.313, 0.156, 0.078, 0.039, 0.020, and 0.098 mg/mL, at a light wavelength of 515 nm. The formula was able to inhibit the Nitric Oxide process with an effective concentration of 1.59 mg/mL. The stability of the medicinal formula was assessed at temperatures of 50°C, 60°C, 70°C, and 80°C, for durations of 3, 6, 9, and 12 days. The results revealed that the formula can be stored at 50°C for 7 days, 60°C for 6 days, 70°C for 4 days, 80°C for 3 days, 25°C for 23 days, and 30°C for 16 days.

Discussion: The study demonstrates that the medicinal formula possesses antioxidant and anti-inflammatory properties, as evidenced by the DPPH assay and Nitric Oxide inhibition method results. These properties align with the traditional use of the formula in alleviating various symptoms, such as chest pain, muscle pain, numbness, loss of taste, and insomnia. Furthermore, the stability study provides valuable information for determining the formula's shelf life and appropriate storage conditions. It is clear that the formula can be stored for different durations at various temperatures, ensuring its efficacy and safety over time.

Conclusion and recommendation: This research provides scientific support for the traditional use of the Thai medicinal formula and highlights its potential benefits in addressing oxidative stress and inflammation, essential aspects of maintaining overall health. Further studies should explore additional variables and extraction methods to provide more comprehensive and accurate results.