The Evaluation of Potentials of Antioxidant Activities, Total Phenolic, Flavonoid, and Tannin Contents from Selected Species in Amanita Crude Extract

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Kritsada Champatasi
Nucharee Chamnantap
Areerat Saisong
Khwanyuruan Khwanyuruan

Abstract

The objective of this study was to evaluate the potential of two common edible species of Amanita, A. princeps Corner & Bas. and A. javanica Corner & Bas. T. Oda, Tanaka & Tsuda, which are local mushrooms of high commercial value. The antioxidant activities were determined by ABTS and FRAP assays, and the amounts of polyphenols (total phenolic, flavonoid compounds, and tannin) were examined in ethanol, methanol, and boiling water crude extracts of the mushrooms. The results showed that the highest percentage yields of 28.97% of total phenolic and flavonoid compounds were found in A. princeps crude ethanolic extract at 18.41 ± 0.13 mg GAE/g extract using a gallic acid standard and 1017.99 ± 8.18 mg QE/g extract with a quercetin standard, while A. princeps crude extract with boiling water had the highest tannin level at 13.53 ± 0.45 mg tannic acid/g extract using a tannic acid standard. Regarding antioxidant activity testing, the two assays showed the highest values with boiling water extract of A. javanica in the ABTS assay, IC50 as 0.75 ± 0.04 mg/mL, while the FRAP assay of ethanolic A. princeps extract recorded 3650.67 ± 35.77 mg FeSO4/g extract.

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References

Sanoamuang N. Wild mushroom of Thailand: biodiversity

and utilization. Bangkok: Universal graphic and

trading. 2010; 424 pp. (in Thai)

Royal Academy. Edible and poisonous mushrooms in

Thailand. The 1st publish, Bangkok: Royal Academy.

; 180 pp. (in Thai)

Jandrasrikul A. Mushroom in Thailand. Bangkok: Thaiwattanapanit

Co.,Ltd.; 1992. 161 pp. (in Thai)

Jandrasrikul A, Suwanarit P, Sangwanit U. Diversity

of mushrooms and macrofungi in Thailand. Bangkok:

Kasatsart University Press; 2008; 514 pp. (in Thai)

Naksuwankul K, Parnmen S, Sikaphan S, Leudang S,

Nooron N, Thongbor A, Chantharasena C. Handbook

of study on morphological characteristics of mushroom,

poisonous mushroom and surveillance. Department of

Medical Sciences, Ministry of Public Health. 2019. 214

pp. (in Thai)

Panmen S, Tongboa A, Jansena C, Sikapan S, Luadung

S, Jantaporn S. Poisonous mushroom manual. Department

of Medical Science, Ministry of Public Health. 2018;

pp. (in Thai)

Sangwanit U, Suwanarit P, Payappanon A, Laungsa-ard

J, Jandrasrikul A, Sakolrak B. Checklist of biodiversity of

mushroom. Biodiversity-Based Economy Development

Office (Public Organization). Bangkok: 2013; 374 pp. (in

Thai)

Department of Thai Traditional and Alternative Medicine,

Ministry of Public Health. Healthy mushroom for

food, medicine, and economy from traditional knowledge

of folk healer. Agricultural cooperative printing demonstrations

of Thai Co., Ltd.; 2013. 408 pp. (in Thai)

Chandrasrikul A. Mushroom in Thailand. Bangkok:

Thaiwatnapanit Co., Ltd.; 1992. 190 pp. (in Thai)

Chandrasrikul A, Suwanarit P, Sangwanit U. Biodiversity

of Mushroom and Macrofungi in Thailand. Bangkok:

Kasetsart University; 2008; 210 pp. (in Thai)

The Royal Institute. Mushroom in Thailand, The Royal

Institute version 2nd publish. Bangkok: Treefilm Co.,

Ltd.; 2007. 120 pp. (in Thai)

Biodiversity-Based Economy Development Office. List

of biological property of microoganism (mushroom).

Bangkok: Auksonsiam Publishing; 2009. 520 pp. (in Thai)

Chandrasrikul A, Suwanarit P, Sangwanit U, Lumyong

S, Payapanon A, Sanoamuang N, Pukahuta C, Petcharat

V, Sardsud U, Duengkae K, Klinhom U, Thongkantha S,

Thongklam S. Checklist of mushrooms (basidiomycetes)

in Thailand: Office of Natural Resources and Environmental

Policy and Planning. Bangkok: 2011. 230 pp.

Sakolrak B, Aryawong J, Duengkae K, Pongpanich K,

Himaman W. Agaris: at Kaeng Krachan forest, Chiang

Dao Wildlife Sanctuary and Phu Khiao Wildlife Sanctuary.

Department of National Parks, Wildlife and Plant

Conservation. Bangkok: 2016. (in Thai)

Rattana S, Sungthong B. Antioxidant activities and

total phenolic contents of methanolic extract from five

fragrant flowers. The 12th Mahasarakham University

Research Conference; Proceeding. 2016. (in Thai)

Daupor H, Saha, A, Chelong A, Meechai A, Waema S.

Determination of total flavonoid content from propolis

stingless bee and bacterial inhibition of Escherichia

coli in soap product. The 6th Science and Technology Conference; Proceeding. Fatoni University Yala. 2017.

(in Thai)

Cham P, Poonsri W, Lichanporn I, Lapsongphon N.

Effects of ripening stages and extraction conditions on

tannin content of “Namwa’’ banana peel powder. The

th National Graduate Research Conference; Proceeding.

; 689-95. (in Thai)

Seekhaw P, Rachinta S, Thurnakul N, Panitan S. Determination

of total phenolic and flavonoid contents,

antioxidant and antibacterial activities of four wild

edible mushrooms from the community market in Loei

Province. PSRU Journal of Science and Technology.

;5(3):60-73. (in Thai)

Satsea S, Thapphasaraphong S, Dumrongrungrua T.

Antioxidant and Collagen Stimulating Activities in

Human Gingival Fibroblast Cell Culture of Plant Extracts.

Isan Journal of Pharmaceutical Science. 2014;(9)

(Suppl.):38-43. (in Thai)

Taweepanit S, Tumkhom P. Antioxidant activity and

total phenolic content of wild edible mushroom extracts

in Ubonratchathani Province. Proceedings UBRC 7th.

27-35. (in Thai)

Phansawan B. Free radicals, antioxidants and antioxidant

activity determination. Journal of Science and

Technology. 2013;21(3):275-86. (in Thai)

Boonsong S, Klaypradit W, Wiliapun P. Antioxidant

activity of extracts from five edible mushrooms using

different extractants. Agriculture and Natural Resources.

;50:89-97.