Quantitative Analysis of Triterpenoids Content in Centella asiatica (L.) Urban. by Ultra Performance Liquid Chromatography (UPLC)
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Abstract
Gotu Kola (Centella asiatica (L.) Urb., Apiaceae) is an herb on the National List of Essential Medicine. The major substances in Gotu Kola is a group of triterpenes and derivatives with various biological activities. The objective of this study is to develop analytical methods for quantification of asiaticoside, madecassoside, asiatic acid and madecassic acid in Gotu Kola raw material and extract using the Ultra Performance Liquid Chromatographic technique by extracting the powdered drug or dissolve the Gotu Kola extract with methanol. A 4 ml – portion of sample solution was injected to UPLC system by using AcquityTM UPLC column BEH C18, 2.1 x 50 mm, 1.7 mm and using distilled water and acetonitrile as mobile phase, flow rate 0.6 ml/min and measured with a PDA at a wavelength of 205 nm. Linearity was established for asiaticoside, asiatic acid, and madecassic acid concentration range of 0.01-0.1 mg/ml and 0.03-0.21 mg/ml for madecassoside with a coefficient of determination (R2) 0.999. The % recovery was in the range of 95-105%. HORRAT was in the range of 0.06-0.3. LOD and LOQ of asiaticoside, madecassoside, asiatic acid and madecassic acid were determined, the LOD were equals 0.0021, 0.0080, 0.0114 and 0.01309 mg/ml, respectively and LOQ were equals 0.0081, 0.0283, 0.0410 and 0.0473 mg/ml, respectively. It was shown that this developed method was suitable and could be used to analyze the content of triterpenoids content in Gotu Kola. The appropriate specification of triterpenoids content in Gotu Kola could be established.
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