The Antioxidant Activities, Total Phenolic, Flavonoid and Melatonin Contents of Five Cultivars of Mulberry Leaves

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Tiantip Kasikorn
Panyada Panyatip
Chawalit Yongram
Orawan Dokkiang
Bunleu Sungthong
Ploenthip Puthongking


The objectives of this study aimed to determine antioxidant activities and analysis total phenolic, flavonoid and melatonin contents of five cultivars of mulberry leaves: Buriram 60, Yai-buriram, Sakonnakhon, Khunpai and Srisaket, were harvested from Queen Sirikit Sericulture Center, Khon Kaen in June, 2018. The results showed that Yai-buriram exhibited the highest antioxidant activity on DPPH (IC50 96.17 ± 2.28 μg/ml) and FRAP (163.99 ± 9.26 mmol/100g DW) assays. While, the ABTS radical scavenging assay indicated that Srisaket comprised a good antioxidant activity with IC50 value of 75.24 ± 1.71 μg/ml. With regards to chemical constituents, Buriram 60 showed the highest flavonoid content at 37.19 ± 0.53 mgQE/g, Yai-Buriram showed the highest phenolic content at 390.89 ± 3.90 mgGAE/g DW and melatonin content at 9.77 ± 0.32 μg/g extract. The result obtained implied that the antioxidant activity of mulberry leaf extracts was related with the amount of total phenolic and flavonoid, and melatonin contents.


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