An Antioxidative and Cytotoxic Substance Extracted from Curcuma comosa Roxb.
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Published:
May 15, 2019
Keywords:
Curcuma comosa antioxidant 4,6-dihydroxy-2-O-(β-D-glucopyranosyl)acetophenone
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Abstract
The powerful antioxidative and HeLa cytotoxic activity of crude ethanol extract of Curcuma comosa Roxb. prompted us to carry out biologically-guided separation of the active constituent. Chromatographic separation of the ethanol extract yielded fractions Zcc(R)-E-1 and Zcc(R)-E-2. The potent fraction Zcc(R)-E- 1 was subsequently bioassay-guided separated and the active fraction Zcc(R)-E-15 was obtained. Recrystallization of fraction Zcc(R)-E-15 yielded compound A, exhibited antioxidant power 1064.73 ± 60.96 μM vitamin C equivalence and HeLa cell cytotoxicity with IC50 4.44 ± 0.85 μg/ml. With structure elucidation via spectroscopic techniques, compound A was identified as 4,6-dihydroxy-2-O-(β-D-glucopyranosyl)acetophenone.
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References
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structure-function studies using acetophenone analogues. European J Pharmacol 2000;387:221-7.
3. Jurgens TM, Frazier EG, Schaeffer JM, Jones TE, Zink DL, Borris RP,et al. Novel nematocidal agents from Curcuma comosa. J Nat Prod 1994;57:230-5.
4. Piyachaturawat P, Ercharuporn S, Suksamrarn A. Uterotropic effect of Curcuma comosa in rats. Int J Pharmacog 1995:33(4):334-8.
5. Piyachaturawat P, Teeratagolpisal N, Toskulkao C, Suksamrarn A.Hypolipidemic effect of Curcuma comosa in mice. Artery 1997;22(5):233-41.
6. Suksamrarn A, Eiamong S, Piyachaturawat P, Byrnre TL. A phloracetophenone glucoside with choleretic activity from Curcuma comosa. Phytochemistry 1997;45:103-5.
7. Piyachaturawat P, Charoenpiboonsin J, Toskulkao C, Suksamrarn A. Reduction of plasma cholesterol by Curcuma comosa extract in hypercholesterolaemic hamster. J Ethnopharmacol 1997;66:199-204.
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9. Schumann K. Zingiberaceae. In: Engler A, editor. Berlin : Pflanzenreich;1903: IV. P. 46.
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version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol 1999;299:15-27.
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