Preliminary Assessment of Phytochemical Content and Antibacterial Activity Against Urinary Tract Pathogens of Herbs in Benjapoltadhu

Main Article Content

James Phungphol
Ploysai Ohama
Thavatchai Kamoltham
Sanjai Sangvichien


Urinary tract infection is a common disease and a significant issue in the Thai public health system. Escherichia coli and Enterococcus faecalis are both major causes of urinary tract infection. Antibacterial resistance is always found in these two pathogens and could lead to difficulty in treatment and may even be life-threatening. The purpose of this study was to screen phytochemical constituents and antibacterial activity of the herbs in Benjapoltadhu, consisting of Cyperus alternifolius L., Homalomena aromatica (Spreng.) Schott, Cyperus rotundus L., Panicum repens L. and Kaempferia galanga L. Extraction of the herbs was carried out by boiling them in water and then freeze drying the extracts. The antimicrobial study was performed against 2 stains, E. coli and E. faecalis using the microdilution method. The results showed that the extract from C. alternifolius L. and H. aromatica (Spreng.) Schott could inhibit E. faecalis with MIC 62.5 and 100.0 mg/ml, respectively. Both herbs were found to contain groups of terpenoids, flavonoids, saponins and tannins. The results suggested that further study is required to elucidate the phytochemical content that possesses antibacterial properties for the development of new medicine in the future.


Download data is not yet available.

Article Details

Original Articles


1. Nopparatana C. Urinary system:urinalysis. 2nd ed. Songkla: Prince of Songkla University; 2013. 197 p. (in Thai)
2. Saensakdi S, Chaichet P, Kingchan S. Prevention of catheter-associated urinary tract infection in orthopedic patients, Khon Kaen Hospital. Journal of Health Science. 2014;23(2):323-34. (in Thai)
3. Monmaturapoj T. Treatment of community-acquired urinary tract infection in the current era of Quinolone resistant. uropathogens. 2014;29(5):475-84. (in Thai)
4. Indrawattana N, Vanaporn M. Nosocomial infection. Journal of medicine and health sciences. 2015;22(1):81-92. (in Thai)
5. Picheansoonthon C, Jeerawong W. Khanapayset. 3rd ed. Bangkok: Amarin printing & publishing; 2013. 350 p. (in Thai)
6. Bunyapraphatsara N, Chokchaichareonporn O, editors. Herbs: folk herbs (1). Bangkok: Faculty of Pharmacy, Mahidol University; 1996. 895 p. (in Thai)
7. Awaad AS, Soliman GA, El-Sayed DF, El-Gindi OD, Alqasoumi.SI. Hepatoprotective activity of Cyperus alternifolius on carbon tetrachloride-induced hepatotoxicity in rats. Pharmaceutical Biology. 2012;50(2):155-61.
8. Bunyapraphatsara N, Chokchaichareonporn O, editors. Herbs: folk herbs (2). Bangkok: Faculty of Pharmacy, Mahidol University; 1998. 640 p. (in Thai)
9. Singh G, Kapoor IPS, Singh OD, Rao GP, Prasad YR, Leclercq PA, Klinkby N. Studies on essential oils, part 28: Chemical composition, antifungal and insecticidal activities of rhizome volatile oil of Homalomena aromatica Schott. Flavour and fragrance journal. 2000;15(4):278-80.
10. Bunyapraphatsara N, Chokchaichareonporn O, editors. Herbs: folk herbs (5). Bangkok: Faculty of Pharmacy, Mahidol University; 2000. 580 p. (in Thai)
11. Lawal OA, Oyedeji AO. Chemical composition of the essential oils of Cyperus rotundus L. from South Africa. Molecules. 2009;14(8):2909-17.
12. Temraz A, Hozaien HE, El-Tantawy WH, El-Gindi OD, Taha KF. Cholestane and spirostane-type glycosides from the roots and rhizomes of Panicum repens L. Phytochemistry letters. 2014;10:173-8.
13. Umar MI, Asmawi MZ, Sadikun A, Altaf R, Iqbal MA. Phytochemistry and medicinal properties of Kaempferia galanga L. (Zingiberaceae) extracts. African Journal of Pharmacy and Pharmacology. 2011;5(14):1638-47.
14. Chatteeranan S, Sabuyjai W, Niyomthai S. Phytochemical screening and antioxidant activity of Clerodendrum disparifolium leaves. KKU science Journal. 2013;41(3):723-30. (in Thai)
15 Temrangsee P. Antibacterial activity of herbal extracts against clinical isolates from wound infection. Pathumthani: Thammasat University; 2011. 100 p. (in Thai)
16. Ranseepanurat W, Chanwittayanuchit I, Pungmaun P, Ngamaurulert S, Chuwongwatana S. Diagnosis bacteria infections in medical. 4th ed. Bangkok: Chulalongkorn University; 2013. 285 p. (in Thai)
17. Suwanpinit N, Suwanpinit P. General microbiology. Bangkok: Chulalongkorn University; 2007. 444 p. (in Thai)
18. Ranseepanurat W, Kummarajadesadakul P, Chanwittayanuchit I. Antibacterial activities of ten Thai herbal extracts against Staphylococcus aureus ATCC 25923, Bacillus cereus and Escherichia coli ATCC 25922. HCU journal of Health science. 2016;19(38):35-48. (in Thai)
19. Zain ME, Awaad AS, Al-Othman MR, Al-Dosary SK. Antibacterial, antifungal activity and phytochemical analysis of some desert plants against human pathogenic bacteria and fungi. Life Science Journal. 2014;11(7):343-9.
20. Barua, CC, Talukdar A, Phukan B, Hazarika S, Barua AG, Baishya G. Phytochemical screening and in vitro antioxidant activity of ethanolic extract of Homalomena aromatica (Araceae) root. Der Pharmacia Lettre. 2014;6(1):128-38.
21. Tantipaibulvut S, Nuamsetti T, Dechayuenyong P. Antibacterial activity of some fruit-peel extracts. KKU Res.J. 2012;17(6):880-94. (in Thai)
22. Tuntrakarnpong R, Tuntrakarnpong J. Antibacterial activity of Ocimum gratissimum L. Crude extract. Sci J of Phetchaburi Rajabhat University. 2013;10(1):40-6. (in Thai)
23. Prasad MP. Analysis of antimicrobial compounds in Cyperus rotundus and Azadirachta indica against human pathogens. Int J Curr Microbiol App Sci. 2014;3(3):206-10.
24. Sini S, Malathy NS. Antimicrobial properties of roots of medicinal plants. Ancient Science of Life. 2005;25(2):62-5.

Most read articles by the same author(s)