Preliminary in Vitro Screening for Antioxidant and Anti-MCF7-Breast Cancer Cell Line Activities of Folk Healers’ Tonic Remedies

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Pariyapat Singthong
Pornprapha Chunthanom
Sasitorn Chusri


This study was aimed to screen the antioxidant and anticancer activities of 15 ethanol extracts of the herbal based folk healers’ tonic remedies used in Sakon Nakhon Thai Traditional Medicine Hospital. The antioxidant activities were assessed by DPPH and FRAP assays. In addition, total phenolic compounds, total flavonoids compounds and total tannin compounds were quantified. Anti-cancer, and cytotoxic properties were evaluated in vitro using MCF7-breast cancer cell line and African green monkey kidney, respectively. The maximum percentage inhibition by DPPH scavenging activity was found to be 181.6 ± 0.9, 71.7 ± 3.9 and 69.9 ± 2.0 from recipe number THS013, THS007 and THS006, respectively. Ferric ion reducing antioxidant power (FRAP) were 3.2 ± 0.0, 3.1 ± 0.1 and 3.0 ± 0.0 mg of ferrous sulphate equivalence/g of extract from recipe number THS008, THS015 and THS013 respectively. The highest total phenolic content was obtained from the extracts of THS015, THS005 and THS009 in the ranged of 3.0–3.4 mg GAE/ml. Total flavonoid content in sample number THS014, THS011 and THS005 was found to be 3.3–4.1 mg CE/ml. The extracts of THS003, THS004 and THS001 possessed the highest tannin content which was in the ranged of 2.2–2.3 mg TAE/ml. Moreover, some of the tonic potent anti-Vero cell from the extracts of THS001 was found to have an IC50 of 18.73 µg/ml, all tonic remedies MCF 7 breast cancer cells were IC50 > 50 µg/ml. The result from this study suggested that these recipes should be studied for their efficacy and safety in detail prior to develop products for health benefits.


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Author Biographies

Pariyapat Singthong, Faculty of Thai Traditional and Alternative Medicine, Ubonratchathani Rajabhat University, Amphoe Mueang, Ubonratchathani 34000, Thailand


Sasitorn Chusri, Faculty of Traditional Thai Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand



1. Slater TF. Free-radical mechanisms in tissue injury. Biochemical Journal. 1984;222(1):1-15.
2. Meghashri S, Chauhan J.B, Zameer F. Contribution of herbal principles towards cytoprotective, antioxidant and anti-Rhizopus activities. South African Journal of Botany. 2012;81:29-33.
3. Limón-Pacheco J, Gonsebatt ME. The role of antioxidants and antioxidant-related enzymes in protective responses to environmentally induced oxidative stress. Mutation Research. 2009;674:137-47.
4. Karadag A, Ozcelik B, Saner S. Review of methods to determine antioxidant capacities. Food Analytical Methods. 2009;2:41-60.
5. Moon J, Shibamoto T. Antioxidant assays for plant and food components. Journal of Agricultural and Food Chemistry. 2009;57:1655-66.
6. Fernández-Sánchez A, Madrigal-Santillán E, Bautista M, Esquivel-Soto J, Morales-González A, Esquivel-Chirino C, Durante-Montiel I, Sánchez-Rivera G, Valadez-Vega C, Morales-González JA. Inflammation, oxidative stress, and obesity. International Journal of Molecular Sciences. 2011;12(5):3117-32.
7. Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Hawkins Byrne D. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. Journal of Food Composition and Analysis. 2006;19:669-75.
8. Samarakoon SM, Chandola HM, Shukla VJ. Evaluation of antioxidant potential of Amalakayas Rasayana: A polyherbal Ayurvedic formulation. International Journal of Ayurveda Research. 2011;2:23-8.
9. Benzie IFF and Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power. The FRAP assay. Anal Biochem. 1996;239(1):70-6.
10. Kaisoon O, Siriamornpun S, Weerapreeyakul N, Meeso N. Phenolic compounds and antioxidant activities of edible flowers from Thailand. Journal of functional food. 2011;2:88-99.
11. Kim DO, Jeong SW, Lee CY. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chemistry. 2003;81:321-6.
12. Hou WC, Lin RD, Cheng KT, Hung YT, Cho CH, Chen CH, Hwang SY, Lee MH. Free radical-scavenging activity of Taiwanese native plants. Phytomedicine. 2003;10(2-3):170-5.
13. Information Technology Division National Cancer Institute Thailand, Hospital-Based Cancer Registry 2015. Pornsup Printing Co., LTD; 2017.
14. Art of healing. Remedies. In Textbook of Thai pharmaceutical. Office of the Permanent Secretary, Ministry of Public Health; n.d. p. 156-73. (in Thai)
15. Wuttummavat W. Encyclopedia of herbs: Thai major pharmaceutical. Bangkok: Odeon Store; 1997. (in Thai)
16. Neamsuvan O, Jaisamut P, Maneenoon K, Subhateerasakul S. A Survey of Medicinal Plants for Tonic from Ban Toong Soong Community Forest, Auluk District, Krabi Province. Burapha Science Journal. 2012;2:160–6.
17. Mishra SL, Sinhamahapatra PK, Nayak A, Das R, Sannigrahi S. In vitro Antioxidant Potential of Different Parts of Oroxylum indicum: A Comparative Study. Indian Journal of Pharmaceutical Sciences. 2010;72(2):267-9.
18. Sithisarn P, Nantateerapong P, Rojsanga P, Sithisarn P. Screening for Antibacterial and Antioxidant Activities and Phytochemical Analysis of Oroxylum indicum Fruit Extracts. Molecules. 2016;21(4):1-8.
19. Pathom Somwonga, Rutt Suttisria, Anumart Buakeawb. New sesquiterpenes and phenolic compound from Ficus foveolata. Elsevier. 2013;85:1–7.
20. Itoh A, Tanaka Y, Nagakura N, Akita T, Nishi T, Tanahashi T. Phenolic and iridoid glycosides from Strychnos axillaris. Phytochemistry. 2008;69:1208-14.
21. Kharat SN, Mendhulkar VD. "Synthesis, characterization and studies on antioxidant activity of silver nanoparticles using Elephantopus scaber leaf extract". Materials Science and Engineering: C Materials for Biological Applications. 2016;62:719-24.
22. Dhanaji M. Ghadagea, Parthraj R. Kshirsagarb, Sandeep R. Paic, Jaykumar J. Chavan. Department. Extraction efficiency, phytochemical profiles and antioxidative properties of different parts of Saptarangi (Salacia chinensis L.)-An important underutilized plant. Biochemistry and Biophysics Reports. 2017;12:79–90.
23. Liu RH. Potential synergy of phytochemicals in cancer prevention: mechanism of action. International Research Conference on Food, Nutrition, and Cancer. 2004;134(12):3479S–85S.
24. Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. The Journal of Nutritional Biochemistry. 2002;13:572–84.
25. Cai YZ, Sun M, Xing J, Luo Q, Corke H. Structure-radical scavenging activity relationships of phenolic compounds from traditional Chinese medicinal plants. Life Sciences. 2006;78:2872-88.
26. Sangmalee S, Laorpaksa A, Sritularak B, Sukrong S, Bioassay-Guided Isolation of Two Flavonoids from Derris scandens with Topoisomerase II Poison Activity. Biological and Pharmaceutical Bulletin. 2016;39:631–5.
27. Wang Z, Sun JB, Qu W, Guan FQ, Li LZ, Liang JY. Caesappin A and B, two novel protosappanins from Caesalpinia sappan L. Fitoterapia. 2014;92:280-4
28. Tao LY, Li JY, Zhang JY. Brazilein, a compound isolated from Caesalpinia sappan Linn., induced growth inhibition in breast cancer cells via involvement of GSK-3β/β-Catenin/cyclin D1 pathway. Chem Biol Interact. 2013;206(1):1-5.