Antibacterial Activity Against Bacillus cereus of Lupinifolin from the Stem of Derris reticulata Craib.

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Kamol Yusook


Bacillus cereus is the bacterium commonly causes food poisoning in human. The contamination of Bacillus cereus in food has been found at a high rate. This study aimed to investigate the antibacterial effects of lupinifolin, a bioactive compound extracted from Derris reticulata against this pathogen. Lupinifolin showed the inhibitory effect in B.cereus growth both on MHB agar cultured-bacteria and Disc diffusion method different from ampicillin (p < 0.05). Furthermore, Microdilution method showed MIC and MBC of the pure compound were 8 and 16 µg/ml, respectively. Under electron microscope, lupinifolin was found to be capable of destroying cell wall and swelling cell membrane of Bacillus cereus. In conclusion, the present results suggests that lupinifolin might exert its anti-B.cereus activity by altering the normal functions of cell wall and cell membrane.Thus, lupinifolin could be a potential compound for the development of antibacterial drugs in the future.


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