Efficiency of Cannabis sativa subsp.sativa Crude Extract On Inhibition Of Pathogenic Bacteria

Abstract

The purpose of this study was to investigate the potential use of crude extract of flowers, leaves and stems from hemp (Cannabis sativa subsp. sativa) to inhibit the growth of Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Klebsiella sp., Acinetobacter spp. and Vibrio cholerae. The samples of hemp; flowers, leaves and stems were extracted using 95% ethanol and then processed using a rotary evaporator to obtain the crude extract. The crude extracts tested against six bacteria using the Agar well diffusion method, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) by Broth Macro dilution. The results showed that the six types of tested bacteria exhibited varying responses to crude extracts from the flowers, leaves, and stems of hemp. Of these extracts, the crude extract from the flowers of hemp was found to be the most effective for inhibiting both gram-positive and gram-negative bacteria, with S.aureus which is gram-positive bacteria being the statistically significant level of 0.05, followed by B. cereus, B. subtilis, Acinetobacter spp., V. cholerae and Klebsiella sp., respectively. The crude extract from the flowers of hemp had a MIC value of 0.0244 mg/ml and a MBC value of 0.0244 mg/ml againstgram-positive bacteria, and a MIC value of 50-12.5 mg/ml and a MBC value of 50 mg/ml against gram-negative bacteria. Therefore, crude extract obtained from different parts of hemp possesses properties for inhibiting pathogenic bacteria. Hemp can be used as a natural alternative extract for the development of antibiotics.