Prevalence of Carbapenemase Genes in Carbapenem non-susceptible Klebsiella pneumoniae isolated from clinical specimens in Buddhachinaraj Phitsanulok Hospital

Abstract

Increasing of carbapenem resistance in Klebsiella pneumoniae affects the drug selection and is a major problem in treating infectious diseases. This study aimed to determine the prevalence of carbapenemase encoding genes in 160 carbapenem non-susceptible K. pneumoniae isolated from clinical specimens in Buddhachinaraj Phitsanulok Hospital during August 2018 to July 2019. Five carbapenemase genes including the blaKPC, blaNDM, blaVIM, blaIMP and blaOXA-48-like were detected by multiplex PCR method. Of 160 isolates, only three resistant genes were found, the prevalences of which were as follow: blaOXA-48-like 80.62 % (129/160), blaNDM 52.50% (84/160) and blaIMP 3.13% (5/160). The blaKPC, and blaVIM genes were not detected. This study revealed a unique prevalence and spread of carbapenemase genes in carbapenemase-producing K. pneumoniae of Buddhachinaraj Phitsanulok Hospital, for guidance in surveillance and prevention of drug resistance.