Efficiency of turmeric germs elimination in the traditional Thai medicine production process with ozone
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Abstract
Background: Herbal medicinal products are accepted in modern medicine circles as alternatives that are increasingly being used to treat disease. It is important to control the quality of raw materials to control the growth of germs in drug production, such as by baking them with low heat at a temperature of 50 degrees Celsius and sending them for irradiation before being sold, which takes time and costs. Therefore, large quantities must be sent in order to cover the operating costs. However, it is found that currently there are studies that use ozone to control the growth of germs in herbal products and the food industry, but no studies have been found on turmeric. This research therefore proposes a method for eliminating germs in herbs and increasing efficiency in the production of herbal medicines with ozone as an alternative to producing standardized Thai traditional medicines.
Objectives: To study the process of germs eliminating by drying the traditional method, we created a turmeric germs elimination device using ozone that does not affect the important substances in the herb for Thai traditional medicine production and studied to compare the amount of the important substance curcumin and the efficiency of germs eliminating in the decontamination process by drying and using ozone.
Methods: Turmeric powder (200 g) was treated with ozone with a production capacity of 60 g/h for a period of 120 minutes in a closed system. Samples of Turmeric powder that was disinfected using ozone were analyzed for the amount of microorganisms (FDA BAM online 2001 (Chapter 3)), yeasts and mold (FDA BAM online 2001 (Chapter 18)). Thin -layer Chromatography was used to indicate the curcumin level with silica gel GF254 for stationary phase and Toluene: chloroform: ethanol (49:49:2) for mobile phase. The curcumin level was investigated in normal light, UV 254 nm, UV 366 nm and Vanillin-sulfuric acid test solution.
Results: Results show that the turmeric powder that has been eliminated by heat treatment (conventional method) found that Microbial levels were at 4.3x102 CFU/g, yeast and mold levels were at 1.4x102 CFU/g, respectively, while turmeric powder that was treated with ozone for 120 minutes was found to have microbial levels at <250EAPC. CFU/g, levels of yeast and mold were <10 CFU/g, respectively. The thin-layer chromatography results were confirmed that ozone treatment in turmeric did not affect the curcumin level.
Conclusions: Ozone treatment is effective to eliminate germs in turmeric powder and does not affect the important substance curcumin in turmeric that will be used in the Thai traditional medicine production.
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กระทรวงสาธารณสุข. ประกาศกระทรวงสาธารณสุข พ.ศ. 2564 เรื่อง เกณฑ์มาตรฐาน ค่าความบริสุทธิ์ หรือคุณลักษณะอื่นอันมีความสำคัญต่อคุณภาพสำหรับตำรับผลิตภัณฑ์สมุนไพรที่ขึ้นทะเบียน แจ้งรายละเอียด หรือจดแจ้งพ.ศ. 2564. ราชกิจจานุเบกษา เล่มที่ 138, ตอนพิเศษ 294 ง (ลงวันที่ 1 ธันวาคม 2564).