Preliminary investigation of phytochemical profiles of honeysuckle grown in Thailand and China
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Abstract
Honeysuckle flowers are considered important medicinal herbs and have been widely used in numerous traditional Chinese medicine formulations. They are employed in the treatment of respiratory disorders, gastrointestinal conditions, and certain dermatological diseases. Thailand currently imports honeysuckle flowers from China in large quantities for use in the pharmaceutical and food industries, While in Thailand, honeysuckle trees are only grown as ornamental plants. This study aimed to extract active compounds from honeysuckle flowers cultivated in Thailand and in China, analyze the physical properties and chemical composition of the extracts; and compare the levels of selected bioactive compounds found in Thai-grown and Chinese-grown honeysuckle flowers, in order to provide a scientific basis for the potential value enhancement of honeysuckle cultivated in Thailand. The findings of the study were as follows: (1) extraction using maceration with hexane, ethyl acetate, methanol, and ethanol solvents revealed that methanol yielded the highest amount of extract, whereas ethyl acetate produced the lowest yield; (2) preliminary phytochemical screening indicated the presence of alkaloids and flavonoids in the extracts. Infrared spectroscopy analysis revealed distinct functional groups as shown in the infrared spectra; and (3) high-performance liquid chromatography (HPLC) analysis of selected bioactive compounds, specifically chlorogenic acid and luteolin, showed that both Thai-grown and Chinese-grown honeysuckle flowers contained these two compounds. However, Chinese-grown honeysuckle had a higher content of chlorogenic acid, while the Thai-grown samples exhibited similar or slightly higher levels of luteolin compared to the Chinese samples.
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