Establishing Root Culture of Cannabis sativa L. for the Production of Active Compounds
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Abstract
Introduction and Objective: Cannabis ( Cannabis sativa L. ) family Cannabiaceae which its roots has been used in treatment of diseases. Disadventage of cannabis cultivation in natural environment are taking a long time less of constitient and contamination microbacterail heavy minerals. The objective of this study is to produced roots using suspensionculture tecnige
Methods: cannabis seeds was sterilized and then culture on MS media until the cotyledon, leaves and roots were appeared explant were culture on 6 different formula media. The roots obtained from suitable media were transform in to the liquid media.
Results: It was found that the most suitable media was 1.25 MS which consisted of growth regulators 6-benzylaminopurine and 1-naphthaleneacetic acid of the concentration 0.5 and 1 mg/L . After 15 days of suspension culture, the weights of roots obtained from cotyledons, leaves and roots were ( mean ± sd ) 0.35 ± 0.36, 0.41 ± 0.15 and 0.40 ± 0.14 grams respectively.
Discussion: Roots suspension culture of cannabis with the above processes and recipes. Reduce heavy metal and microbial contamination and increase root load quickly. Also found freidelin.
Conclusion and Recommendation: In The future, it will be used for the lage scale productory for future pharmacological study.
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