Screening for Determination of Immunomodulatory Activities of Medicinal Plants Using Lymphocyte Proliferation Assay Detecting Immunomodulating Activities of Medicinal Plant
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Abstract
The objective of this study was to screen Thai medicinal plant extracts for lymphoproliferative activities in peripheral blood mononuclear cells. Seventeen medicinal plant extracts were tested for lymphocyte proliferation
in vitro assay, using the carboxyfluorescein succinimidyl ester (CFSE) for intracellular labelling of lymphocytes and tracking the cell proliferation with the flow cytometer. The proliferative cells were detected by progressive reduction in CFSE fluorescence intensity. It was found that water extracts of Helicteres isora L., Mesosphaerum suaveolens (L.) Kuntze, could stimulate the lymphocyte proliferation, while Kaempferia parviflora Wallich ex Baker inhibited lymphocyte proliferation in vitro. The screening tool for detecting immunomodulating activities of Thai medicinal plants could identify the properties of extracts that lead to the development of Thai traditional medicines to further promote treatment and stimulate immunity with Thai herbs.
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