A Study of Antioxidant and Physicochemical Stability in Antipyretic Decoction of Traditional Lanna Recipe

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Sakoontala Kongdee
Ratchadawan Aukkanimart
Jirunya Promchiang
Areeya Changthong
Pranee Sriraj

Abstract

During the current COVID-19 outbreak, Thai traditional antipyretic recipes and antipyretic herbs have been widely studied. Antipyretic Thai/Lanna herbal decoction for relieving fever in the North consists of Tinospora crispa, Rhinacanthus nasutus, Sauropus androgynus and Alstonia scholaris. This research aimed to study the physical stability, pH, sedimentation, and color of the Lanna antipyretic decoction at 4°C, 25°C and 40°C with 75±5% RH for 7 days. The testing included the Folin Ciocalteu method for quantifying total phenolic content, the aluminium chloride colorimetry for flavonoid content, and the DPPH radical scavenging and ABTS methods for antioxidant activities. The results demonstrated that, for the decoction, the pH values at various temperatures dropped with storage time, the sediment amounts increased with storage time, but its color did not change at 4°C. Its total phenolic and flavonoid contents rose with storage time. The total phenolic contents in mg GAE/g extract at Days 0 and 7 were 161.13±5.62 and 15.34±6.21 at 4°C, 160.53±11.78 and 14.32±1.13 at 25°C, and 160.09±7.39 and 12.82±6.91 at 40°C, respectively; while the flavonoid contents in mgQE/g extract at Days 0 and 7 were 20.32±0.43 and 7.80±0.34 at 4°C, 20.10±0.47 and 7.27±0.20 at 25°C, and 20.06±0.55 and 6.63±0.43 at 40°, respectively. The decoction’s free radical inhibition (IC50) values at 4°, 25° and 40°C by DPPH ranged from 14.74±0.04 to 23.18±0.72 %w/w; and by ABTS the values ranged from 2.63±0.12 to 3.95±0.05 %w/w. At the storage temperature of 4°C, the decoction showed the best physical stability. Thus, the results can be used in setting up storage guidelines for Thai traditional and folk decoctions to ensue the best stability.

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References

Sawai W, Daranee O. A study of medicinal plants utilization of folk healers: a case study of folk healers in Chiang Rai Province Thailand. J Thai Trad Alt Med. 2018;16(3):420-35. (in Thai)

Suraphan S. Treatment of fever with the philosophical theory of Thai traditional medicine. [Internet]. 2014 [cited 2020 Dec 20]; Available from: http://www.morsuraphan.com/knowledge/64. (in Thai)

Yingyong T, Kanyanoot T. Tamra kanphaet phưnban Lanna sakha mohya: choose kanphaet phưnban lae kanphaet chonpao paknai series. Chiang Rai: Thailand. School of Traditional and Alternative Medicine, Chiang Rai Rajabhat University; 2004. 166 p. (in Thai)

Sudpranorm S, Worawan K. Pathophysiology in nursing. Bangkok: Srisavarindhira Thai Red Cross Institute of Nursing; 2004. 84 p. (in Thai)

Hou C-C, Lin H, Chang C-P, Huang W-T, Lin M-T. Oxidative stress and pyrogenic fever pathogenesis. Eur J Pharmacol. 2011;667(1-3):6-12.

Langrand J, Regnault H, Cachet X, Bouzidi C, Villa AF, Serfaty L, Garnier R, Michel S. Toxic hepatitis induced by a herbal medicine: Tinospora crispa. Phytomedicine. 2014;21(8-9):1120-3.

Lai RS, Chiang AA, Wu MT, Wang JS, Lai NS, Lu JY, Ger LP, Roggli V. Outbreak of bronchiolitis obliterans associated with consumption of Sauropus androgynus in Taiwan. Lancet. 1996;348(9020):83–5.

Zhao Y-L, Su M, Shang J-H, Wang X, Bao G-L, Ma J, Sun Q-D, Yuan F, Wang J-K, Luo X-D. Acute and sub-chronic toxicity of indole alkaloids extract from leaves of Alstonia scholaris (L.) R. Br. in beagle dogs. Nat Prod Bioprospect. 2020;10(4):209-20.

Puangkaew N, Udompittayasan J, Sukdang K, Rattanama P, Hlong N. A study of herbal folk medicine wisdom to treat the flu: case study of Mr. Jitr Boonluang, Wang Wiset district, Trang province. TJPHS. 2021;4(2):111-22. (in Thai)

Raymond CR, Paul JS, Siân CO. Handbook of pharmaceutical excipients. 5th editions. Royal Pharmaceutical Society of Great Britain London, UK: Pharmaceutical press and American pharmacists association; 2006. 651 p.

Ayurveda College (Jivaka Komarapat). Thai pharmacy textbook. 2nd editions. Bangkok: Thai Traditional Medicine. Promotion Foundation; 2559. 262 p. (in Thai)

Joungmunkong Z, Na-Ranong S, Rakrod S, Ornlaor S, Sila-on W. Accelerated stability testing of extemporaneous suspensions and reconstituted powder for suspensions of acetazolamide and furosemide. Isan J Pharm Sci. 2013;9(1):32-9. (in Thai)

Mohamed El-Sayed M, Metwally NH, Ibrahim IA, Abdel-Hady H, Abdel-Wahab BSA. Antioxidant activity, total phenolic and flavonoid contents of Petroselinum crispum Mill. J Appl Life Sci Int. 2018;19(2):1-7. (in Thai)

Liyana-Pathirana CM, Shahidi F. Antioxidant activity of commercial soft and hard wheat (Triticum aestivum L.) as affected by gastric pH conditions. J Agric Food Chem. 2005;53(7):2433–40.

Oyedemi SO, Bradley G, Afolayan AJ. In vitro and in vivo antioxidant activities of aqueous extract of Strychonos henningsii Gilg. Afr. J Pharm Pharmacol. 2010;4(2):70–8.

Haque M, Jantan I, Harikrishnan H, Ahmad W. Standardized ethanol extract of Tinospora crispa upregulates pro-inflammatory mediators release in LPS-primed U937 human macrophages through stimulation of MAPK, NF-κB and PI3K-Akt signaling networks. BMC Complement Med Ther. 2020;20(1):245.

Rakib A, Ahmed S, Islam MA, Haye A, Uddin SMN, Uddin MMN, Hossain MK, Paul A, Emran TB. Antipyretic and hepatoprotective potential of Tinospora crispa and investigation of possible lead compounds through in silico approaches. Food Sci Nutr. 2020;8(1):547-56.

Panichayupakaranant P, Charoonratana T, Sirikatitham A. RP-HPLC analysis of rhinacanthins in Rhinacanthus nasutus: validation and application for the preparation of rhinacanthin high-yielding extract. J Chromatogr Sci. 2009;1;47(8):705-8.

Bose R, Kumar MS, Manivel A, Chandra Mohan S. Chemical constituents of Sauropus androgynus and evaluation of its antioxidant activity. Res. J. Phytochem. 2018;12(1):7-13.

Pankti K, Payal G, Manodeep C, Jagadish K. A phytopharmacological review of Alstonia scholaris: A panoramic herbal medicine. Int J Ayurveda Res. 2012;3(3):367-71.

Weerapreeyakul N, Sripanidkulchai B, Chadbunchachai S, Subcharoen P. Evaluation of physical properties of Benjakul decoction and the possibility of contamination with heavy metals. J Thai Trad Alt Med. 2007;5(2):142-50. (in Thai)

Kubicek CP, Esser K, Druzhinina IS. Environmental and microbial relationships. Vol. 4. Heidelberg, Germany: Springer Science & Business Media; 2007.

Sila-on W, Sungkapat S, Anansuchartkul J, Wattanawong W. Physical and chemical stability study of morphine sulfate syrup. Isan J Pharm Sci. 2008;4(2):134-9. (in Thai)

Ritdet P, Masthong R, Limsuwan S, Sakulkeo O, Joycharat N. Physical and chemical stability of glycerin formulations containing extracts from Albizia myriophylla and cha-em-thang song. Isan J Pharm Sci. 2020;16(2):45-56. (in Thai)

Ounamornmas P, Sommano S. Analyses of bioactive ingredients and antioxidant activities of some edible flowers. Journal of Agriculture (Thailand). 2016;32(3):435-45. (in Thai)

Elmegerhi S, Su C, Buglewicz DJ, Aizawa Y, Kato TA. Effect of hydroxyl group position in flavonoids on inducing single‑stranded DNA damage mediated by cupric ions. Int J Mol Med. 2018;42(1):658-64.

Chaaban H, Ioannou I, Chebil L, Slimane M, Gerardin C, Paris C, Charbonnel C, Chekir L, Ghoul M. Effect of heat processing on thermal stability and antioxidant activity of six flavonoids. J Food Process Preserv. 2017;41(5):e13203.

Alam F, Saqib QNU. Pharmacognostic study and development of quality control parameters for fruit, bark and leaf of Zanthoxylum armatum (Rutaceae). Anc Sci Life. 2015;34(3):147-55.