Bioactivities of Leucaena leucocephala Young Leaf Crude Extract

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Rungkarn Sangkaruk
Pawaret Panyajai

Abstract

Leucaena leucocephala (krathin in Thai) has been used in foods and traditional medicines for a long time. This study aimed to assess L. leucocephala young leaf extract for determining its total phenolic content, investigate the inhibition of Heinz body formation, hemolytic effect and protection of G6PD-deficient human erythrocytes against oxidative damage, antimicrobial activity, and cytotoxicity on leukemic cell lines. The results demonstrated that the total phenolic content of L. leucocephala young leaf extract was 325.6 ± 0.7 mg gallic acid equivalent per gram of dried extract (mg GAE/g extract). The extract could inhibit the Heinz body formation induced by acetylphenylhydrazine (APH) and prevent hemolysis damage induced by hydrogen peroxide in a concentration-dependent manner. The highest inhibition of Heinz body formation and protection values were about 12.8% and 4.3%, respectively, at the concentration 1,000 g/mL for G6PD-deficient erythrocytes. Regarding toxicity, KG-1a cell was more sensitive to the extract than Molt4 cell. The IC20 of extract on KG-1a cell was 79.2 ± 0.5 μg/mL, but the extract had no antimicrobial effect on bacteria from clinical samples. It is thus suggested that further in vivo studies should conducted on the effect of L. leucocephala young leaf extract to determine its non-toxic therapeutic doses for preventing oxidative stress and hemolysis in G6PD-deficient erythrocytes.

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References

Salem A.F.Z, Salem M.Z, Gonzalez-Ronquillo M, Camacho L.M., Cipriano M. Major chemical constituents of Leucaena leucocephala and Salix babylonica leaf extracts. Journal of Tropical Agriculture. 2011;49:95-8.

Gamal-eldeen A.M, Amer H, Helmy W.A, Ragab H.M, Talaat R.M. Antiproliferative and cancer-chemopreventive properties of sulfated glycosylated extract derived from Leucaena leucocephala. Indian J. Pharm. Sci. 2007;69(6):805-11.

Aderibigbe S.A, Adetunji O.A, Odeniyi M. A. Antimicrobial and pharmaceutical properties of the seed oil of Leucaena leucocephala (Lam.) De Wit (Leguminosae). Afr. J. Biomed. Res. 2011;14:63-8.

Chowtivannakul P, Srichaikul B, Talubmook C. Antidiabetic and antioxidant activities of seed extract from Leucaena leucocephala (Lam.) de Wit. Agriculture and Natural Resources. 2016;50(5):357-61.

Chung H.H, Chen M.K, Chang Y.C, Yang S.F, Lin C.C, Lin C.W. Inhibitory effects of Leucaena leucocephala on the metastasis and invasion of human oral cancer cells. Environmental Toxicology. 2017;32(6):1765-74.

Hassan R.A, Tawfik W.A, Abou-Setta L.M. The flavonoid constitunts of Leucaena leucocephala. Growing in Egypt, and their biological activity. Afr J Tradit Complement Altern Med. 2014;11(1):67-72.

Palasuwan A, Soogarun S, Lertlum T, Pradniwat P, Wiwanitkit V. Inhibition of Heinz body induction in an in vitro model and total antioxidant activity of medicinal Thai plants. Asian Pac J Cancer Prev. 2005;6(4):458-63.

Dükel M, Tavsan Z, Ayar Kayali H. Flavonoids regulate cell death-related cellular signaling via ROS in human colon cancer cells. Process Biochemistry. 2021;101:11-25.

Zarin M.A, Wan H.Y, Isha A, Armania N. Antioxidant, antimicrobial and cytotoxic potential of condensed tannins from Leucaena leucocephala hybrid-Rendang. Food Science and Human Wellness. 2016;5(2):65-75.

Nilsson R, Liu N.A. Nuclear DNA damages generated by reactive oxygen molecules (ROS) under oxidative stress and their relevance to human cancers, including ionizing radiation-induced neoplasia part I: Physical, chemical and molecular biology aspects. Radiation Medicine and Protection. 2020;1(3):140-52.

Harris I.S, DeNicola G.M. The complex interplay between antioxidants and ROS in cancer. Trends Cell Biol. 2020 Jun;30(6):440-51

Luzzatto L, Ally M, Notaro R. Glucose-6-phosphate dehydrogenase deficiency. Blood. 2020;136(11):1225-40.

Georgakouli K, Deli C.K, Zalavras A, Fatouros I.G, Kouretas D, Koutedakis Y, Jamurtas A.Z. α-lipoic acid supplementation up-regulates antioxidant capacity in adults with G6PD deficiency. Food Chem Toxicol. 2013;61:69-73

Juliusson G, Hough R. Leukemia. Prog Tumor Res. 2016;43:87-100.

Spinelli R, Guevara L.A.B, López J.A, Camargo C.M, Restrepo H.G, Siano S.A. Cytotoxic and antiproliferative activities of amphibian (anuran) skin extracts on human acute monocytic leukemia cells. Toxicon. 2020;177:25-34.

John B, Sulaiman CT, George S, Reddy V.R.K. Total phenolics and flavonoids in selected medicinal plants from Kerala. Int J Pharm Pharm Sci. 2014;6(1):406-8.

Ghosh T, Biswas MK, Chatterjee S. In-vitro study on the hemolytic activity of different extracts of Indian medicinal plant Croton bonplandianum with phytochemical estimation: a new era in drug development. Journal of Drug Delivery and Therapeutics. 2018;8(4):155-60.

Ahmed B, Hashmi A, Khan M.S, Musarrat J. ROS mediated destruction of cell membrane, growth and biofilms of human bacterial pathogens by stable metallic AgNPs functionalized from bell pepper extract and quercetin. Advanced Powder Technology. 2018;29(7):1601-16.

Kuznetsov A.V, Javadov S, Saks V, Margreiter R, Grimm M. Synchronism in mitochondrial ROS flashes, membrane depolarization and calcium sparks in human carcinoma cells. Biochim Biophys Acta Bioenerg. 2017;1858(6):418-31.

Al-Jaber N.A, Awaad A.S, Moses J.E. Review on some antioxidant plants growing in Arab world. Journal of Saudi Chemical Society. 2011;15(4):293-307.

Abboud M.M, Al-Awaida W. Synchrony of G6PD activity and RBC fragility under oxidative stress exerted at normal and G6PD deficiency. Clin Biochem. 2010;43(4-5):455-60

Lin C.J, Ho H.Y, Cheng M, Cheng M.L, You T.H, Yu J.S, Chiu D.T.Y. Impaired dephosphorylation renders G6PD-knockdown HepG2 cells more susceptible to H2O2-induced apoptosis. Free Radic Biol Med. 2010;49(3):361-73.

Rizzello C.G, Losito I, Facchini L, Katina K, Palmisano F, Gobbetti M, Coda R. Degradation of vicine, convicine and their aglycones during fermentation of faba bean flour. Sci Rep. 2016;6:32452.

Bubp J, Jen M, Matuszewski K. Caring for Glucose-6-Phosphate Dehydrogenase (G6PD)–Deficient Patients: Implications for Pharmacy. P T. 2015;40(9):572–4.

Zayed M.Z, Samling B. Phytochemical constituents of the leaves of Leucaena leucocephala from Malaysia. Int J Pharm Pharm Sci. 2016;8(12):174-9.

Haggag E.G, Kamal A.M, Abdelhady M.I.S, El-Sayed M.M, El-Wakil E.A, Abd-El-Hamed SS. Antioxidant and cytotoxic activity of polyphenolic compounds isolated from the leaves of Leucenia leucocephala. Pharm Biol. 2011;49(11):1103-13.

Yin L, Han H, Zheng X, Wang G, Li Y, Wang W. Flavonoids analysis and antioxidant, antimicrobial, and anti-inflammatory activities of crude and purified extracts from Veronicastrum latifolium. Industrial Crops and Products. 2019;137:652-61.

Gutiérrez-Venegas G, Gómez-Mora J.A, Meraz-Rodríguez M.A, Flores-Sánchez M.A, Laura Ortiz-Miranda F. Effect of flavonoids on antimicrobial activity of microorganisms present in dental plaque. Heliyon. 2019;5(12):e03013.

Mohammed R.S, Souda S.S.E, Taie H.A.A, Moharam M.E, Shaker K.H. Antioxidant, antimicrobial activities of flavonoids glycoside from Leucaena leucocephala leaves. Journal of Applied Pharmaceutical Science. 2015;5(6):138-47.

Bisignano C, Ginestra G, Smeriglio A, Camera E.L, Crisafi G, Franchina F.A, Tranchida P.Q, Alibrandi A, Trombetta D, Mondello L, Mandalari G. Study of the lipid profile of ATCC and clinical strains of Staphylococcus aureus in relation to their antibiotic resistance. Molecules. 2019;24(7):1276.

Honda M.D.H, Borthakur D. Mimosine is a stress-response molecule that serves as both an antioxidant and osmolyte in giant leucaena (Leucaena leucocephala subsp. glabrata) during environmental stress conditions. Plant Stress. 2021;2:100015