Method Development and Validation for Quantification of Rhinacanthin-C in Rhinacanthus nasutus Roots

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Salinee Na Ranong
Prapapun Sukphan
Jiranuch Jamtaweekul


The quantity of a chemical constituent in a herb is a good indicator for quality assessment as the constituent can indicate that the herb has enough active component to ensure its efficacy, or whether it is toxic. Therefore, the researchers developed an analytical method for quantifying the amount of rhinacanthin-C, which is a major chemical constituent found in the roots of Rhinacanthus nasutus (thongphanchang in Thai) by using the High-Performance Liquid Chromatography (HPLC) technique. This method was validated for its specificity, linearity, precision and accuracy. The method could separate rhinacanthin-C from other chemical components in the sample of R. nasutus roots. The validation of this analytical method showed that the linearity was between 0.04 and 0.20 mg/mL and the correlation coefficient value was 0.9995. The precision and intermediate precision tests showed %RSD of 0.39% and 1.39%, respectively. The accuracy test showed %recovery at low, medium and high concentration levels of 97.6%, 97.5% and 97.4%, respectively. The results suggest that the developed method can be used to quantify the amount of rhinacanthin-C in raw R. nasutus roots.


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