Isolation of Pinostrobin, a Chemical Marker from Fingerroots for Quality Control Purposes

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Duangpen Pattamadilok
Apirak Sakpetch

Abstract

Pinostrobin is a major constituent of fingerroot or krachai in Thai [Boesenbergia rotunda (L.) Mansf.]. From
preliminary study, isolation of high-purity pinostrobin could be possible. This compound will be used as a chemical marker in method development for quality control of fingerroot for which no standard method had been established for this medicinal plant in Thailand. The aims of this study were to study the isolation method of pinostrobin from fingerroot and to develop a chemical identification method of fingerroot using the thin layer chromatography (TLC) technique. Isolation of pinostrobin was performed by maceration of fingerroot with 95% ethanol and evaporation of the filtrate to gain yellow precipitate. Sephadex LH20 gel filtration was performed and pale yellow powder was obtained. Recrystallization was carried on to obtain off-white powder. Structural identification of the isolated compound was undertaken using spectroscopic analysis. A TLC method using pinostrobin as a chemical marker was developed. Sample was prepared by extracting fingerroot powder with methanol, using silica gel 60 as stationary phase and a mixture of dichloromethane and methanol (70:1) as mobile phase, and then sprayed with NP/PEG. Results showed that 97.06% pure pinostrobin could be isolated with 0.86% yield. Using this uncomplicated
method, the high-purity and high-yield compound could be obtained. In the TLC analysis, a spot of pinostrobin at the Rf value of 0.95 was observed. This method could be further used for quality control of fingerroot.

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