Anti-inflammatory Effect of Ya-Mahanintangtong Remedy Extract in RAW 264.7 Cell Line

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Bhanuz Dechayont
Pathompong Phuaklee
Jitpisute Chunthorng-Orn
Onkamon Jampa
Thana Juckmeta
Kitrawee Jiraratsatit


Ya-Mahanintangtong is a Thai herbal remedy listed in the National List of Essential Medicine 2018. According to Thai traditional medicine, this drug recipe possesses anti-infective effects and is used for the treatment of fever, pharyngitis and chickenpox. However, up till now there has been no scientific report regarding the efficacy and safety of Ya-Mahanintangtong remedy itself. Therefore, the objective of this study was to assess the anti-inflammatory effect of Ya-Mahanintangtong remedy. Thus, proinflammatory cytokines, tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) production were analyzed using ELISA. The result obtained indicated that the ethanolic extract exhibited higher inhibitory activity TNF-α than IL-6 with IC50 values of 33.09 ± 0.22 and 17.20 ± 0.65 µg/mL, respectively. This work demonstrated the anti-inflammatory effect of Ya-Mahanintangtong remedy in vitro which preliminarily support the use of this Thai folk medicine as an antipyretic and anti-inflammatory agent. However, additional evidence on its safety and efficacy is required. Both preclinical and clinical studies are necessary to ensure the use of this recipe clinically.


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